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首页> 外文期刊>Journal of dairy science >Metabolic characteristics of proteins and biomolecular spectroscopic profiles in different batches of feedstock (wheat) and their co-products (wheat distillers dried grains with solubles) from the same bioethanol processing plant
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Metabolic characteristics of proteins and biomolecular spectroscopic profiles in different batches of feedstock (wheat) and their co-products (wheat distillers dried grains with solubles) from the same bioethanol processing plant

机译：来自同一生物乙醇加工厂的不同批次的原料（小麦）及其副产品（小麦蒸馏器干燥的谷物和可溶物）中蛋白质的代谢特征和生物分子光谱图

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摘要

The objectives of this study were (1) to reveal the metabolic characteristics of proteins in different batches of feedstock (wheat) for bioethanol production and their co-products (wheat distillers dried grains with solubles, wDDGS) from the same bioethanol processing plant, and (2) to characterize biomolecular spectral profile associated with nutrient digestion in the rumen and intestine of dairy cattle. The metabolic characteristics of proteins were determined using the DVE/OEB system (where DVE = total truly absorbed protein supply, and OEB = degraded balance of protein) based on chemical profiles and rumen and intestinal digestion data from dairy cattle. The biomolecular spectral characteristics were investigated by using the molecular spectroscopy technique attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FT/IR). Multivariate molecular spectral analyses-agglomera-tive hierarchical cluster analysis (AHCA), and principal component analysis (PCA)-were conducted to identify the spectral differences in biomolecular inherent structure among the wheat and wDDGS batches. The results showed that (1) the metabolic characteristics of proteins in the wheat and wDDGS from the same bioethanol processing plant were significantly affected by batch, with total truly absorbed protein supply (DVE value) ranging from 101 to 116 g/kg of dry matter (DM) in wheat and from 153 to 182 g/kg of DM in wDDGS; (2) the degraded balance of protein (OEB value) in the wDDGS (but not the wheat) from the same bioethanol processing plant was significantly affected by batch, with the OEB value ranging from -19 to -26 g/kg of DM in the wheat and from 145 to 181 g/kg of DM in the wDDGS; and (3) the biomolecular spectral analyses with AHCA and PCA revealed biomolecular spectral profiles and differences among the wheat and wDDGS samples.
机译：这项研究的目的是（1）揭示同一生物乙醇加工厂中用于生产生物乙醇的不同批次原料（小麦）中的蛋白质及其副产品（小麦蒸馏器含可溶物的干谷物，wDDGS）的代谢特性，以及（2）表征奶牛瘤胃和肠道中与营养物质消化有关的生物分子光谱图。蛋白质的代谢特征使用DVE / OEB系统（其中DVE =真正吸收的总蛋白质供应量，而OEB =蛋白质的平衡降低量）基于奶牛的化学特征和瘤胃和肠道消化数据确定。通过使用分子光谱技术衰减全反射-傅里叶变换红外光谱（ATR-FT / IR）研究了生物分子的光谱特性。进行了多元分子光谱分析-聚类层次聚类分析（AHCA）和主成分分析（PCA）-以确定小麦和wDDGS批次之间生物分子固有结构的光谱差异。结果表明：（1）同一批生物乙醇加工厂的小麦和wDDGS中蛋白质的代谢特征受批次的影响很大，真正吸收的蛋白质总供应量（DVE值）为101至116 g / kg干物质。 （DM）在小麦中，而wDDGS中的DM从153至182 g / kg （2）同一批生物乙醇加工厂的wDDGS（而不是小麦）中的蛋白质降解平衡（OEB值）受到批次的显着影响，在DM中，OEB值介于-19至-26 g / kg DM之间。小麦和wDDGS中的DM含量为145至181 g / kg （3）用AHCA和PCA进行的生物分子光谱分析揭示了小麦和wDDGS样品之间的生物分子光谱特征和差异。

著录项

  • 来源
    《Journal of dairy science》 |2012年第11期|6695-6715|共21页
  • 作者

    Inoka H. Gamage; Arjan Jonker; David A. Christensen; Peiqiang Yu;

  • 作者单位

    College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8;

    College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8;

    College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8;

    College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bioethanol feedstock and co-products; metabolic characteristics of proteins; biomolecular; spectral profile;

    机译：生物乙醇原料和副产品;蛋白质的代谢特征;生物分子光谱轮廓;

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