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首页> 外文期刊>Journal of dairy science >Short communication: Characterization of Shiga toxin 2-carrying bacteriophages induced from Shiga-toxigenic Escherichia coli isolated from Italian dairy products
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Short communication: Characterization of Shiga toxin 2-carrying bacteriophages induced from Shiga-toxigenic Escherichia coli isolated from Italian dairy products

机译：简短交流：从意大利乳制品中分离出的志贺毒素大肠埃希菌诱导的携带志贺毒素2的噬菌体的表征

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摘要

Forty samples of raw milk cheese and 25 samples of raw milk itself were subjected to enrichment culture for Shiga-toxigenic Escherichia coli (STEC), and a single Shiga toxin 2- (Stx_2) positive strain was obtained from one of the cheese samples. Thus, aged cheeses in which the curd is subsequently heat treated (48℃) cannot be presumed to be STEC free. Infective Stx_2 bacteriophages were induced from 3 STEC strains isolated elsewhere from raw milk and 1 STEC strain from aged cheese sourced in Italy. Data on E. coli host range, morphology, genome size, and genetic variation determined by restriction fragment length polymorphism and multi-locus genotyping are presented. Although all 4 bacteriophages were found to be short-tailed Podo-viridae, they exhibited considerable variation in both genome size and content. This extended to the Stx_2 genes themselves, whose sequences contained several point mutations, but these did not translate to amino acid substitutions.
机译：对40个生乳奶酪样品和25个生乳本身样品进行了志贺毒素大肠杆菌（STEC）的富集培养，并且从其中一个奶酪样品中获得了一个志贺毒素2-（Stx_2）阳性菌株。因此，不能将其中凝乳随后进行了热处理（48℃）的陈年奶酪假定为不含STEC。感染性Stx_2噬菌体是从在其他地方从生奶中分离出的3株STEC菌株和从意大利采购的陈旧奶酪中分离出的1株STEC菌株诱导而来。介绍了有关大肠杆菌宿主范围，形态，基因组大小和遗传变异的数据，这些数据由限制性片段长度多态性和多基因座基因分型确定。尽管发现所有4种噬菌体均为短尾Podo病毒科，但它们在基因组大小和含量上均表现出相当大的差异。这扩展到Stx_2基因本身，其序列包含几个点突变，但是这些并不能翻译为氨基酸取代。

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  • 来源
    《Journal of dairy science》 |2012年第12期|6949-6956|共8页
  • 作者

    G. Volponi; D. J. Rooks; D. L. Smith; C. Picozzi; H. E. Allison; I. Vigentini; R. Foschino; A. J. McCarthy;

  • 作者单位

    Dipartmento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2 20133 Milano, Italy;

    Microbiology Research Group, Institute of Integrative Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom;

    Microbiology Research Group, Institute of Integrative Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom;

    Dipartmento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2 20133 Milano, Italy;

    Microbiology Research Group, Institute of Integrative Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom;

    Dipartmento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2 20133 Milano, Italy;

    Dipartmento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi di Milano, via Celoria 2 20133 Milano, Italy;

    Microbiology Research Group, Institute of Integrative Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    shiga-toxigenic escherichia coli; milk and cheese; shiga toxin bacteriophage; protected designation of origin dairy product;

    机译：滋贺毒素大肠埃希菌;牛奶和奶酪;志贺毒素噬菌体;受保护的原产地标记;

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