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首页> 外文期刊>Journal of dairy science >Effects of ruminal ammonia and butyrate concentrations on reticuloruminal epithelial blood flow and volatile fatty acid absorption kinetics under washed reticulorumen conditions in lactating dairy cows
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Effects of ruminal ammonia and butyrate concentrations on reticuloruminal epithelial blood flow and volatile fatty acid absorption kinetics under washed reticulorumen conditions in lactating dairy cows

机译：乳牛泌乳过程中瘤胃氨和丁酸浓度对网状上皮血流和挥发性脂肪酸吸收动力学的影响

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The effect of reticuloruminal epithelial blood flow on the absorption of propionate as a volatile fatty acid (VFA) marker in 8 lactating Holstein cows was studied under washed rumen conditions. The cows were surgically prepared with ruminal cannulas and permanent catheters in an artery and mesenteric, right ruminal, and hepatic portal veins. The experiment was designed with 2 groups of cows: 4 cows adapted to high crude protein (CP) and 4 to low CP. All cows were subjected to 3 buffers: butyric, ammonia, and control in a randomized replicated 3×3 incomplete Latin square design. The buffers (30 kg) were maintained in a temporarily emptied and washed rumen for 40 min. The initial concentration of VFA was 84.2 mmol/L. Butyrate was increased from 4 to 36 mmol/L in butyric buffer by replacement of acetate, and ammonia (NH_3) was increased from 2.5 to 22.5 mmol/L in ammonia buffer by replacement of NaCl. Increasing amounts of deuterium oxide (D_2O) were added to the buffers as the order of buffer sequence increased (6, 12, and 18 g of D_2O). Ruminal clearance of D_2O was used to estimate epithelial blood flow. To increase accuracy of the epithelial blood flow estimates, data of ruminal liquid marker (Cr-EDTA), and initial and final buffer volumes were fitted to a dynamic simulation model. The model was used to estimate ruminal liquid passages, residual liquid, and water influx (saliva and epithelia water) for each combination of cow and buffer (n = 24). Epithelial blood flow increased 49 ± 11% for butyric buffer compared with control. The ruminal disappearance of propionate (marker VFA) was affected by buffer and followed the same pattern as for epithelial blood flow. The correlation between ruminal disappearance of propionate and epithelial blood flow (r = 0.56) indicates that the removal of propionate can be limited by epithelial blood flow. The ruminal disappearance of propionate increased 30 ± 12% for the butyric compared with ammonia buffer and 12.5 ± 8% when compared with control. The net portal flux of propionate increased 32 ± 6% in butyric compared with control. In conclusion, rumen epithelial blood flow is positively correlated with ruminal disappearance of propionate and affects the kinetics of ruminal VFA absorption.%Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, DK-8830 Tjele, Denmark;Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061;Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, DK-8830 Tjele, Denmark;
机译：在清洗的瘤胃条件下，研究了8只泌乳荷斯坦奶牛网状上皮血流对丙酸作为挥发性脂肪酸（VFA）标记的吸收的影响。用瘤胃插管和永久导管在动脉和肠系膜，右瘤胃和肝门静脉中进行手术准备。实验设计了2组母牛：4头适应高粗蛋白（CP）的母牛和4头适应低CP的母牛。所有奶牛均接受3种缓冲液：丁酸，氨水和对照，采用随机复制的3×3不完全拉丁方形设计。将缓冲液（30kg）保持在暂时排空并洗涤的瘤胃中40分钟。 VFA的初始浓度为84.2 mmol / L。通过替换乙酸盐，在丁酸缓冲液中将丁酸盐从4增加到36 mmol / L，通过替换NaCl，将氨气（NH_3）从在氨气缓冲液中从2.5增加到22.5 mmol / L。随着缓冲液顺序的增加（6、12和18 g D_2O），将越来越多的氧化氘（D_2O）添加到缓冲液中。 D_2O的瘤胃清除率用于估计上皮血流量。为了提高上皮血流估计的准确性，将瘤胃液标记物（Cr-EDTA）的数据以及初始和最终缓冲液体积拟合到动态模拟模型中。该模型用于估计奶牛和缓冲液（n = 24）的每种组合的瘤胃液体通道，残留液体和水流入量（唾液和上皮水）。与对照相比，丁酸缓冲液的上皮血流量增加了49±11％。丙酸的瘤胃消失（标记VFA）受缓冲液影响，并遵循与上皮血流相同的模式。瘤胃的丙酸消失与上皮血流之间的相关性（r = 0.56）表明丙酸的去除可能受到上皮血流的限制。与氨水相比，丁酸的瘤胃丙酸瘤胃消失增加30±12％，而与对照组相比，则增加12.5±8％。与对照组相比，丁酸中丙酸盐的净门户通量增加了32±6％。总之，瘤胃上皮的血流量与丙酸瘤胃的消失呈正相关，并影响瘤胃VFA吸收的动力学。％，丹麦奥尔胡斯大学农业科学学院动物健康与生物科学系，DK-8830，丹麦杰勒；乳业系弗吉尼亚理工学院和州立大学自然科学系，布莱克斯堡24061；奥尔胡斯大学农业科学学院动物健康与生物科学系，丹麦特里DK-8830；

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