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首页> 外文期刊>Journal of dairy science >Effects of supplementary folic acid and vitamin B_(12) on hepatic metabolism of dairy cows according to methionine supply
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Effects of supplementary folic acid and vitamin B_(12) on hepatic metabolism of dairy cows according to methionine supply

机译：叶酸和蛋氨酸的补充对叶酸和维生素B_（12）的补充对奶牛肝脏代谢的影响

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摘要

The present experiment was undertaken to study the interactions between dietary supplements of rumen-protected methionine (RPM) and intramuscular injections of folic acid and vitamin B_(12), given from 3 wk before calving to 16 wk of lactation, on hepatic metabolism of lactating dairy cows. Sixty multiparous Holstein cows were assigned to 10 blocks of 6 cows each according to their previous milk production. Within each block, 3 cows were fed a diet calculated to supply Met as 1.83% of metabolizable protein, whereas the 3 other cows were fed the same diet supplemented with 18 g of RPM calculated to provide Met as 2.23% of metabolizable protein. Within each level of Met, the cows received no vitamin supplement or weekly intramuscular injections of 160 mg of folic acid alone or combined with 10 mg of vitamin B_(12). Liver biopsies were taken at 2, 4, 8, and 16 wk of lactation. Liver concentrations of folates and vitamin B_(12) were increased by their respective supplements but this response to vitamin supplements was altered by methionine supply. Concentrations of total lipids and triglycerides increased in livers of cows fed RPM, whereas concentrations of cholesterol ester, cholesterol, diglycerides, phosphatidylethanolamine, and phosphatidylcholine were not affected. Folic acid, alone or combined with vitamin B_(12), tended to increase the ratio of phosphatidylcholine to phosphatidylethanolamine. Gene expression of 5,10-methylene-tetrahy-drofolate reductase, microsomal transfer protein, and phosphatidylethanolamine methyltransferase were higher in liver of cows fed RPM supplements. The relative mRNA abundance of 5,10-methylene-tetrahydro-folate reductase and methylmalonyl-CoA mutase werernincreased by the combined injections of folic acid and vitamin B_(12), whereas those of methionine synthase and methionine synthase reductase were not affected by treatments. These results suggest that increasing supply of methyl groups, as preformed labile methyl groups or through methylneogenesis, affected the methylation cycle but had a limited effect on dairy cow performance. The observed effects of the combined supplement of folic acid and vitamin B_(12) on lactational performance of dairy cows probably result from an improvement of energy metabolism during early lactation.
机译：本实验的目的是研究日粮中添加瘤胃保护的蛋氨酸（RPM）与肌内注射叶酸和维生素B_（12）之间的相互作用（从泌乳前3周到泌乳16周），以了解哺乳期肝脏的代谢奶牛。根据他们以前的产奶量，将60头荷斯坦牛分为10头，每头6头。在每个区块中，给3头母牛饲喂经计算可提供1.83％可代谢蛋白质的Met的日粮，而向其他3头母牛饲喂相同的膳食，并补充18 g RPM（经计算可提供2.23％的可代谢蛋白质的Met）。在每个Met水平内，奶牛均未补充维生素或每周肌注160 mg叶酸或与10 mg维生素B_（12）肌肉注射。在哺乳期第2、4、8和16周进行肝活检。叶酸和维生素B_（12）的肝脏浓度通过各自补充而增加，但是对蛋氨酸补充的这种反应因蛋氨酸的供应而改变。饲喂RPM的奶牛肝脏中总脂质和甘油三酸酯的浓度增加，而胆固醇酯，胆固醇，甘油二酸酯，磷脂酰乙醇胺和磷脂酰胆碱的浓度不受影响。叶酸单独或与维生素B_（12）结合使用，往往会增加磷脂酰胆碱与磷脂酰乙醇胺的比例。补充RPM的奶牛肝脏中5,10-亚甲基-四氢-叶酸还原酶，微粒体转移蛋白和磷脂酰乙醇胺甲基转移酶的基因表达较高。叶酸和维生素B_（12）的联合注射增加了5,10-亚甲基四氢叶酸还原酶和甲基丙二酰辅酶A突变酶的相对mRNA丰度，而蛋氨酸合酶和蛋氨酸合酶还原酶的相对mRNA丰度却不受治疗的影响。这些结果表明，如预先形成的不稳定甲基或通过甲基新生成作用，甲基供应的增加会影响甲基化周期，但对奶牛生产性能的影响有限。观察到叶酸和维生素B_（12）的联合补充对奶牛泌乳性能的影响可能是由于早期泌乳期间能量代谢的改善。

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  • 来源
    《Journal of dairy science》 |2010年第5期|p.2130-2142|共13页
  • 作者

    A. Preynat; H. Lapierre; M. C. Thivierge; M. F. Palin; N. Cardinault; J. J. Matte; A. Desrochers; C. L. Girard;

  • 作者单位

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

    Departement de Sciences Animales, Universite Laval, Quebec, G1V 0A6, Canada Rowett Institute of Health and Nutrition, Aberdeen University Aberdeen, United Kingdom AB21 9SB;

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

    Faculte de Medecine Veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, J2S 7C6, Canada;

    Agriculture et Agroalimentaire Canada, Centre de Recherche et Developpement sur le Bovin Laitier et le Porc, Sherbrooke, Quebec, J1M 1Z3, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    dairy cow; folic acid; vitamin B_(12); rumen-protected methionine;

    机译：奶牛;叶酸;维生素B_（12）;瘤胃保护的蛋氨酸;

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