• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dairy science >In vitro digestibility of individual amino acids in rumen-undegraded protein:The modified three-step procedure and the immobilized digestive enzyme assay
【24h】

In vitro digestibility of individual amino acids in rumen-undegraded protein:The modified three-step procedure and the immobilized digestive enzyme assay

机译：瘤胃未降解蛋白质中单个氨基酸的体外消化率：改进的三步法和固定化消化酶测定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Three soybean meal, 3 SoyPlus (West Central Cooperative, Ralston, IA), 5 distillers dried grains with solubles, and 5 fish meal samples were used to evaluate the modified 3-step in vitro procedure (TSP) and the in vitro immobilized digestive enzyme assay (IDEA; Novus International Inc., St. Louis, MO) for estimating digestibility of AA in rumen-undegraded protein (RUP-AA). In a previous experiment, each sample was ruminally incubated in situ for 16 h, and in vivo digestibility of AA in the intact samples and in the rumen-undegraded residues (RUR) was obtained for all samples using the precision-fed cecectomized rooster assay. For the modified TSP, 5 g of RUR was weighed into polyester bags, which were then heat-sealed and placed into Daisy incubator bottles. Samples were incubated in a pepsin/HCI solution followed by incubation in a pancreatin solution. After this incubation, residues remaining in the bags were analyzed for AA, and digestibility of RUP-AA was calculated based on disappearance from the bags. In vitro RUP-AA digestibility estimates obtained with this procedure were highly correlated to in vivo estimates. Corresponding intact feeds were also analyzed via the pepsin/pancre-atin steps of the modified TSP. In vitro estimates of AA digestibility of the feeds were highly correlated to in vivo RUP-AA digestibility, which suggests that the feeds may not need to be ruminally incubated before determining RUP-AA digestibility in vitro. The RURrnwere also analyzed via the IDEA kits. The IDEA values of the RUR were good predictors of RUP-AA digestibility in soybean meal, SoyPlus, and distillers dried grains with solubles, but the IDEA values were not as good predictors of RUP-AA digestibility in fish meal. However, the IDEA values of intact feed samples were also determined and were highly correlated to in vivo RUP-AA digestibility for all feed types, suggesting that the IDEA value of intact feeds may be a better predictor of RUP-AA digestibility than the IDEA value of the RUR. In conclusion, the modified TSP and IDEA kits are good approaches for estimating RUP-AA digestibility in soybean meal products, distillers dried grains with solubles, and fish meal samples.
机译：使用3份豆粕，3份SoyPlus（美国爱荷华州拉尔斯顿的West Central Cooperative），5份带有可溶物的蒸馏酒干燥谷物和5份鱼粉样品来评估改良的3步体外操作程序（TSP）和体外固定化消化酶检测方法（IDEA；美国密苏里州圣路易斯的Novus International Inc.）用于评估瘤胃未降解蛋白（RUP-AA）中AA的消化率。在先前的实验中，将每个样品在瘤胃中原位温育16小时，并使用精密喂养的切除公鸡方法对所有样品的完整样品和瘤胃未降解残留物（RUR）中的AA进行体内消化。对于改良的TSP，将5克RUR称入聚酯袋中，然后将其热封并放入雏菊培养箱中。将样品在胃蛋白酶/ HCl溶液中孵育，然后在胰酶溶液中孵育。孵育后，分析残留在袋子中的残留物的AA，并根据袋子中的消失量计算RUP-AA的消化率。通过该程序获得的体外RUP-AA消化率估计值与体内估计值高度相关。还通过修饰的TSP的胃蛋白酶/胰蛋白酶步骤分析了相应的完整饲料。饲料中AA消化率的体外估计值与体内RUP-AA消化率高度相关，这表明在体外确定RUP-AA消化率之前，无需对饲料进行反刍动物孵化。还通过IDEA套件分析了RURrn。 RUR的IDEA值可以很好地预测豆粕，SoyPlus和带有可溶物的酒糟中RUP-AA的消化率，但是IDEA值不能很好地预测鱼粉中RUP-AA的消化率。但是，还确定了完整饲料样品的IDEA值，并且与所有饲料类型的体内RUP-AA消化率高度相关，这表明完整饲料的IDEA值可能比IDEA值更好地预测RUP-AA消化率RUR。总之，经过改进的TSP和IDEA试剂盒是估算豆粕产品，带有可溶物的酒糟和酒粕样品中RUP-AA消化率的好方法。

著录项

  • 来源
    《Journal of dairy science》 |2009年第8期|3939-3950|共12页
  • 作者

    S. E. Boucher; S. Calsamiglia; C. M. Parsons; M. D. Stern; M. Ruiz Moreno; M. Vazquez-Anon; C. G. Schwab;

  • 作者单位

    Department of Biological Sciences, University of New Hampshire, Durham 03824 William H. Miner Agricultural Research Institute, Chazy, NY 12921;

    Facultad de Veterinaria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain;

    Department of Animal Sciences, University of Illinois, Champaign 61801;

    Department of Animal Science, University of Minnesota, St. Paul 55108;

    Department of Animal Science, University of Minnesota, St. Paul 55108;

    Novus International Inc., St. Louis, MO 63147;

    Department of Biological Sciences, University of New Hampshire, Durham 03824;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    amino acid digestibility; modified three-step procedure; immobilized digestive enzyme assay;

    机译：氨基酸消化率修改的三步程序;固定化消化酶测定;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号