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首页> 外文期刊>Journal of ecotoxicology & environmental monitoring >ASSESSMENT OF EMBRYOGENIC CALLI DERIVED FROM TEMPERATURE SENSITIVE GENIC MALE STERILITY BASED RICE HYBRIDS FOR SALT TOLERANCE
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ASSESSMENT OF EMBRYOGENIC CALLI DERIVED FROM TEMPERATURE SENSITIVE GENIC MALE STERILITY BASED RICE HYBRIDS FOR SALT TOLERANCE

机译：基于温度敏感性基因型雄性不育基于水稻杂种的耐盐性胚芽钙的评估

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摘要

Five temperature sensitive genie male sterile (TGMS) lines viz., GD 98028, GD 98029, GD 98021, GD 98168 and GD 98179, eight salt tolerant testers viz., CSR 10, CSR 23, CSSRI 13, CSSRI 60, Nona Bokra, Vytilla 3, TRY 2 and CO 43 and 40 hybrids obtained by crossing them in Line x Tester design were screened for their salt tolerance under in vitro conditions. Earlier callus induction, higher percentage of callus induction and maximum fresh weight of callus was obtained when rice embryos were inoculated and cultured in MS media supplemented with 2.4-D 2 mg l~(-1), kinetin 0.25 g l~(-1) and casein hydrolysate 1 g l~(-1). The media were utilized in screening of parents and hybrids for salt tolerance in five varied concentrations of NaCl (0.0,0.4,0.8,1.2 & 1.6 %). The genotypes were ranked based on the criteria callus morphology score and fresh weight of callus recorded after three weeks of screening. The hybrid GD 98029/CSSRI 13 ranked first followed by the hybrids GD 98028/CO 43, GD 98028/CSR 23, GD 98029/CSR 10, GD 98029/Nona Bokra and the parent Nona Bokra in MS media supplemented with 2,4-D 2 mg l~(-1), kinetin 0.25 g l~(-1) and casein hydrolysate 1 g l~(-1) along with NaCl at 1.6 per cent concentration exhibiting their potential for salt tolerance.
机译：五个温度敏感的雄性不育系（TGMS），即GD 98028，GD 98029，GD 98021，GD 98168和GD 98179，八个耐盐性测试仪，即CSR 10，CSR 23，CSSRI 13，CSSRI 60，Nona Bokra，通过在Line x Tester设计中杂交获得的Vytilla 3，TRY 2和CO 43和40个杂种，在体外条件下筛选了它们的耐盐性。在添加2.4-D 2 mg l〜（-1），激动素0.25 gl〜（-1）和酪蛋白水解物1 gl〜（-1）。该培养基用于筛选五种不同浓度的NaCl（0.0、0.4、0.8、1.2和1.6％）中的耐盐性亲本和杂种。根据标准的愈伤组织形态学评分和筛选三周后记录的新鲜愈伤组织重量对基因型进行排名。杂种GD 98029 / CSSRI 13位居第二，其次是杂种GD 98028 / CO 43，GD 98028 / CSR 23，GD 98029 / CSR 10，GD 98029 / Nona Bokra和母本Nona Bokra在MS培养基中补充2,4- D 2 mg l〜（-1），激动素0.25 gl〜（-1）和酪蛋白水解物1 gl〜（-1）以及浓度为1.6％的NaCl表现出其耐盐性。

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