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首页> 外文期刊>Journal of Virology >Intracellular Forms of Adenovirus Deoxyribonucleic Acid I. Evidence for a Deoxyribonucleic Acid-Protein Complex in Baby Hamster Kidney Cells Infected with Adenovirus Type 12
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Intracellular Forms of Adenovirus Deoxyribonucleic Acid I. Evidence for a Deoxyribonucleic Acid-Protein Complex in Baby Hamster Kidney Cells Infected with Adenovirus Type 12

机译：腺病毒脱氧核糖核酸的细胞内形式。婴儿仓鼠肾细胞中脱氧核糖核酸蛋白复合物的证据12型感染腺病毒12型

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摘要

The total intracellular deoxyribonucleic acid (DNA) from baby hamster kidney cells abortively infected with 3H-adenovirus type 12 was analyzed in dye-buoyant density gradients. Between 10 and 20% of the cell-associated radioactivity derived from viral DNA bands in a density position which is 0.043 to 0.085 g/cm3 higher than that of viral DNA extracted from purified virions. The DNA in the high-density region (HP-fraction) is almost completely absent when DNA, ribonucleic acid (RNA) or protein synthesis is chemically inhibited in separate experiments. The HP-fraction is not found when the virus does not adsorb to and enter the cell. The DNA in the HP-fraction appears as early as 2 hr after inoculation. At 2 hr after infection, the HP-fraction is present both in the nucleus and the cytoplasm. This DNA hybridizes exclusively with viral DNA and sediments at approximately the same rate in both neutral and alkaline sucrose density gradients. Electron microscopy has revealed no circular DNA molecules in this fraction. Evidence indicates that the viral DNA in the HP-fraction exists in a complex with protein and possibly RNA. The protein component of the complex is resistant to enzymatic digestion, whereas the complex is susceptible to ribonuclease treatment. Digestion with deoxyribonuclease reduces the amount of DNA found in the HP-fraction. The structure and biological function of this complex are currently being investigated.
机译：在染料浮力密度梯度中分析来自婴儿仓鼠肾细胞的细胞内脱氧核糖核酸（DNA）的总细胞内脱氧核糖核酸（DNA）。在浓度位置的衍生自病毒DNA条带的10至20％的细胞相关放射性之间，其为0.043至0.085g / cm 3的高于从纯化的病毒粒子中提取的病毒DNA的0.043-0.085g / cm。当DNA，核糖核酸（RNA）或蛋白质合成在单独的实验中化学抑制时，高密度区域（HP级别）中的DNA几乎完全不存在。当病毒未吸附并进入细胞时，未发现HP-F部分。 HP-Fract中的DNA在接种后早等为2小时。在感染后2小时，在细胞核和细胞质中存在HP馏分。该DNA仅在中性和碱性蔗糖密度梯度上以大致相同的速率与病毒DNA和沉积物杂交。电子显微镜显示在该级分中没有圆形DNA分子。证据表明，HP级分中的病毒DNA存在于蛋白质和可能的RNA中的复合物中。复合物的蛋白质组分耐酶消化，而复合物易于核糖核酸酶处理。用脱氧核酸核酸酶消化减少了HP级分中发现的DNA量。目前正在研究该综合体的结构和生物学功能。

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