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首页> 外文期刊>Infection and immunity >In Vitro Studies on Rickettsia-Host Cell Interactions: Intracellular Growth Cycle of Virulent and Attenuated Rickettsia prowazeki in Chicken Embryo Cells in Slide Chamber Cultures
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In Vitro Studies on Rickettsia-Host Cell Interactions: Intracellular Growth Cycle of Virulent and Attenuated Rickettsia prowazeki in Chicken Embryo Cells in Slide Chamber Cultures

机译：体外研究Rickettia-宿主细胞相互作用：毒株毒力和衰减的Rickettsia prowazeki的细胞内生长循环在湿室培养中的鸡胚细胞中

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Upon entry into the cytoplasm of irradiated chicken embryo cells in slide chamber cultures infected over a 2-h period, yolk sac-grown virulent (Breinl strain) and attenuated (E strain) Rickettsia prowazeki underwent indistinguishable reproducible intracellular growth cycles. They promptly entered an exponential growth phase, without detectable lag and without microscopic evidence for any unusual early replicative phase. The generation time for both strains was 8.8 to 8.9 h at 34 C. During most of this period, the state of the organisms and growth were very similar from one cell to another. The exponential-growth phase continued for at least 36 to 48 h, when the rickettsiae became too numerous to count by microscopic examination. Between about 36 and 48 h, cells packed with rickettsiae began irregularly to break down and release organisms. These began to initiate new infection cycles in previously uninfected cells over many hours, as demonstrated by the rise in percentage of cells infected, yielding a highly disordered infected culture with different cells containing rickettsiae in diverse stages of growth. The organisms underwent regular minor changes in morphology, similar to those seen in bacterial cultures, in the first infection cycle. As the cells became packed with rickettsiae, the microorganisms regularly diminished in size to become minute coccobacillary to coccoid forms. However, the rickettsiae in the second and subsequent infection cycles in aging cultures often assumed filamentous or swollen bizarre forms. Only the first infection cycle conformed closely to the concept of a one-step growth cycle. A set of terms is proposed and defined for the infection cycle.
机译：在进入辐射的鸡胚细胞的辐射室培养物中，蛋黄囊生长（Breinl菌株）和减毒（E菌株） Rickettsia prowazeki 接受了难以区分的细胞内生长循环。他们迅速进入指数增长阶段，没有可检测的滞后，没有显微透视任何不寻常的早期复制阶段。 34℃的两种菌株的生成时间为8.8至8.9小时。在此期间的大部分期间，生物体和生长的状态与一个细胞与另一个细胞非常相似。当Rickettiae因微观检查而变得太多时，指数增长阶段持续至少36至48小时。在约36到48小时之间，用Rickettsia填充的细胞不规则地分解和释放生物。这些开始在多小时内开始在前未感染的细胞中发起新的感染循环，如感染细胞的百分比上升所示，产生高度无序的受感染培养物，其具有含有Rickettiae的不同细胞的生长阶段。生物体经常在第一感染循环中经常进行常规的形态变化，类似于细菌培养物中的那些。由于细胞与Rickettiae填充，微生物的尺寸定期降低，以使小茧状成分为Coccoid形式。然而，在衰老培养物中的第二个和随后的感染循环中的Rickettsiae经常假定丝状或肿胀的奇异形式。只有第一感染周期符合一步生长周期的概念。提出了一系列术语并为感染周期定义。

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