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首页> 外文期刊>Journal of bacteriology >Evidence that the recA441 (tif-1) mutant of Escherichia coli K-12 contains a thermosensitive intragenic suppressor of RecA constitutive protease activity.
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Evidence that the recA441 (tif-1) mutant of Escherichia coli K-12 contains a thermosensitive intragenic suppressor of RecA constitutive protease activity.

机译:证据表明,大肠杆菌K-12的RECA411(TIF-1)突变体含有RECA构成蛋白酶酶活性的热敏腺体抑制剂。

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摘要

The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in recA441, which is at codon 298, appears to be acting as a temperature-sensitive suppressor of the protease activity.
机译:已知已被认为具有热宜素的组成型RECA蛋白酶活性的大肠杆菌的RECA411突变体在RECA内具有两个突变。我们在此显示改变密码子38的突变实际上赋予温度无关的本构型蛋白酶酶活性;在密码子298处的RECA441中的第二个突变似乎用作蛋白酶活性的温度敏感的抑制剂。

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