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首页> 外文期刊>Infection and immunity >Relationship of genotype of recombinants of influenza A/Hong Kong/68-ts-1[E]virus used as live virus vaccines to virulence in humans.
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Relationship of genotype of recombinants of influenza A/Hong Kong/68-ts-1[E]virus used as live virus vaccines to virulence in humans.

机译：甲型/香港/香港/香港/ 68-TS-1 [E]病毒重组基因型的关系用作人类病毒疫苗对人类毒力的影响。

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Influenza A/Hong Kong/68-ts-1[E] virus is a temperature-sensitive mutant developed for use as a live virus vaccine (B. R. Murphy, E. G. Chalhub, S. R. Nusinoff, J. Kasel, and R. M. Chanock, J. Infect. Dis. 128:479--487, 1973). This virus and temperature-sensitive recombinants derived by mating it with A/Udorn/72, A/Georgia/74, or A/Victoria/75 wild-type virus have been administered to volunteers in clinical trials on the assumption that the ts-1[E] temperature-sensitive genetic lesions on a polymerase gene (P3) and on the nucleoprotein gene (NP) would determine a satisfactory and reproducible level of attentuation regardless of the genetic constitution of ts-1[E] recombinants at other loci (B. R. Murphy, D. D. Richman, S. B. Spring, and R. M. Chanock, Postgrad. Med. 52:381--388, 1976). In this paper, the parental origin of genes in the ts-1[E] recombinants was determined by using the technique of polyacrylamide gel electrophoresis of virion ribonucleic acid segments in the presence of a denaturing agent (urea). When tested in individuals who lacked immunity to hemagglutinin antigen, attenuation of the ts-1[E] recombinants appeared to correlate with inheritance of the ts-1[E] temperature-sensitive genes at the P3 and NP loci and with the level of preinfection neuraminidase immunity. There was no evidence that other genes from the ts-1[E] donor virus played a role in attenuation.
机译：流感A /香港/ 68-TS-1 [e]病毒是一种用于现场病毒疫苗的温度敏感突变体（BR Murphy，例如Chalhub，SR Nusinoff，J. Chanock，J.Cycect 。DIS。128：479--487,1973）。通过将其与A / Udorn / 72，A / Georgia / 74或/ Victoria / 75或/ Victoria / 75野生型病毒联系起来的这种病毒和温度敏感的重组剂已经向临床试验中的志愿者施用，假设TS-1 [E]聚合酶基因（P3）和核蛋白基因（NP）上的温度敏感遗传病变将确定令人满意和可重复的病情水平，无论在其他基因座的TS-1 [E]重组剂的遗传结构如何（BR墨菲，DD Richman，SB Spring和RM Chanock，Postgrad。Med。52：381--388,1976）。在本文中，通过在变性剂（尿素）存在下，通过使用维罗氏核糖核酸段的聚丙烯酰胺凝胶电泳技术来确定TS-1 [E]重组中基因的父母来源。当在缺乏对血凝素抗原缺乏免疫的个体中测试时，TS-1 [E]重组剂的衰减似乎与P3和NP基因座的TS-1 [E]温度敏感基因的遗传相关，并且具有预缩合水平神经氨酸酶免疫。没有证据表明TS-1 [E]供体病毒的其他基因在衰减中起作用。

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