• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Immunological properties of Escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity.
【24h】

Immunological properties of Escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity.

机译：大肠杆菌热稳定肠毒素的免疫学特性：乳胶小鼠活性的热稳定肠毒素特异性的放射免疫分析。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antiserum was raised against the purified heat-stable enterotoxin (ST) produced by enterotoxigenic Escherichia coli strain 431, a class II porcine enteropathogen. The antiserum was used to examine the antigenic determinants of STs produced by enterotoxigenic strains of different host origins and develop a sensitive radioimmunoassay specific for ST having biological activity in suckling mice and piglets (STA). The antiserum neutralized one effective dose of toxin at a dilution of 1:5,000 and neutralized approximately 40 microgram of toxin per ml of serum. In the radioimmunoassay, protein A-bearing staphylococci was used as the primary solid-phase adsorbent. The purified STs produced by a class I enteropathogen (strain 667) and by a bovine enterotoxigenic strain (B-41) exhibited patterns of competitive inhibition identical to those of homologous unlabeled strain 431 ST in the radioimmunoassay when specific antibody to strain 431 ST was used. The levels of ST in culture supernatants determined by the suckling mouse assay correlated with the concentrations of toxin measured by the radioimmunoassay. The antiserum was specific for STA produced by enterotoxigenic E. coli of porcine, bovine, and human origins and did not react with heat-labile enterotoxin or with ST that had biological activity in piglets but not in suckling mice (STB). These results suggest that STA molecules having different host origins share at least one antigenic determinant.
机译：抗血清针对由肠毒素大肠杆菌菌株431，II类猪肠病理学产生的纯化的热稳定肠毒素（ST）。抗血清用于检查不同宿主起源的肠毒素菌株产生的STS的抗原决定因素，并在哺乳小鼠和仔猪（STA）中形成具有生物活性的ST的敏感放射免疫测定。抗血清以1：5,000的稀释液中和一种有效剂量的毒素，并用每毫升血清中和约40微克毒素。在放射免疫测定中，用蛋白质α-含蛋白葡萄球菌作为初级固相吸附剂。由I类肠病（菌株667）和通过牛肠肠菌株（B-41）制备的纯化的STS表现出与在使用特异性菌株431ST的特异性抗体时，与放射免疫测定中的同源未标记菌株431S相同的竞争性抑制模式。由幼儿小鼠测定测定的ST在培养上清液中的水平与通过放射免疫测定测量的毒素的浓度相关。抗血清特异于猪，牛和人类起源的肠毒素大肠杆菌生产的STA，并且与热稳定肠毒素或与仔猪生物活性的ST没有反应，但不与哺乳小鼠（STB）反应。这些结果表明，具有不同宿主起源的STA分子共有至少一种抗原决定簇。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号