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首页> 外文期刊>Journal of bacteriology >Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.
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Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.

机译：400碱基对细胞COLE2-P9的400碱基对片段中基因的表达足以引起宿主细胞裂解。

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摘要

The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,000) and two smaller proteins (molecular weights, 5,000 and 3,000), and lysed when incubated in medium containing isopropyl-beta-D-thiogalactopyranoside (IPTG). A 400-base-pair lacp-distal fragment derived from the insert in this plasmid was recloned in the same orientation into pUR222. Although hosts carrying this plasmid also lysed when grown in the presence of IPTG, they were sensitive to colicin E2 and produced increased amounts of the 5,000- and 3,000-molecular-weight proteins (but not the full-length immunity protein) when treated with IPTG. The results were consistent with the idea that expression of celB (production of the 5,000- and 3,000-molecular-weight proteins) is sufficient to cause host cell lysis in the absence of colicin production and derepression of the host cell SOS system.
机译：在高拷贝数质粒pUR222中的LAC启动子的控制下，在LAC启动子的控制下克隆了肠道质粒COLE2-P9的肠道e2抗扰度（CEIB）和裂解（CELB）基因。携带该质粒的宿主免受肠道E2免疫，产生增加的免疫蛋白（分子量，9,000）和两个较小的蛋白质（分子量，5,000和3,000），并在含有异丙基-D-硫代酰键酰胺（ iptg）。在该质粒中衍生自插入物的400碱基对LAC-远端片段以与pUR222相同的取向沉入。虽然携带这种质粒的宿主在IPTG存在下生长时也裂解，但它们对肠溶蛋白E2敏感，并且当用IPTG处理时，它们增加了5,000-和3,000分子量蛋白（但不是全长免疫蛋白）的量增加。结果与CELB的表达（5,000-和3,000分子量蛋白质的产生）一致的结果一致地使宿主细胞裂解在没有肠道生产和宿主细胞SOS系统的情况下。

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