...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >A physical map of the viral genome for infectious pancreatic necrosis virus Sp: analysis of cell-free translation products derived from viral cDNA clones.
【24h】

A physical map of the viral genome for infectious pancreatic necrosis virus Sp: analysis of cell-free translation products derived from viral cDNA clones.

机译：传染性胰腺坏死病毒病毒基因组的物理图谱SP：源自病毒cDNA克隆的无细胞翻译产品分析。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The two segments of double-stranded RNA from infectious pancreatic necrosis virus Sp were cloned into the plasmid vector pUC8. Two sets of overlapping clones were identified by restriction enzyme and Southern blot analyses. Each of these sets was shown by Northern blot analysis to be exclusively related to either segment A or B of the genomic RNA. The entire lengths of the cloned segments were estimated to be 2.9 and 2.6 kilobases, respectively. Sequences from the two segments of viral cDNA were subcloned into the bacteriophage T7 RNA polymerase vectors pT71 and pT72. The activity of the single-stranded RNAs transcribed from these subclones in a rabbit reticulocyte lysate translation system provided information on the polarity of and the protein products coded for by each subclone. The four proteins encoded by the genome of infectious pancreatic necrosis virus were identified among the translation products of the individual cloned segments by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. By constructing plasmids containing deletions in the sequences from either the 5' or 3' end of segment A, we were able to construct a physical map for the larger segment of double-stranded RNA. The proteins derived from these plasmids indicated that the linear gene order for viral proteins encoded in segment A is beta, gamma 2, and gamma 1.
机译：来自传染性胰腺坏死病毒Sp的双链RNA的两段克隆到质粒载体pUC8中。通过限制酶和Southern印迹分析鉴定两组重叠克隆。通过Northern印迹分析显示这些组中的每一种，以与基因组RNA的一段A或B级相关。将克隆段的整个长度分别估计为2.9和2.6千千碱基。将来自病毒cDNA的两段的序列亚克隆到噬菌体T7 RNA聚合酶PT71和PT72中。在兔网卵细胞翻译系统中转录的单链RNA的活性提供了关于每个亚克隆编码的极性和蛋白质产品的信息。通过免疫沉淀和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，鉴定了由传染性胰腺坏死病毒的基因组编码的四种蛋白质。通过构建含有序列中的序列中的缺失的质粒，我们能够构建用于较大的双链RNA片段的物理图。源自这些质粒的蛋白质表明，在β，γ2和γ1中编码的病毒蛋白的线性基因序列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号