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Microbial community composition of a multi-stage moving bed biofilm reactor and its interaction with kinetic model parameters estimation

机译:多级移动床生物膜反应器的微生物群落组成及其与动力学模型参数估计的相互作用

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摘要

Microbial community diversity determines the function of each chamber of multi-stage moving bed biofilm reactor (MBBR) systems. How the microbial community data can be further used to serve wastewater treatment process modelling and optimization has been rarely studied. In this study, a MBBR system was set up to investigate the microbial community diversity of biofilm in each functional chamber. The compositions of microbial community of biofilm from different chambers of MBBR were quantified by high-throughput sequencing. Significantly higher proportion of autotrophs were found in the second aerobic chamber (15.4%), while 4.3% autotrophs were found in the first aerobic chamber. Autotrophs in anoxic chamber were negligible. Moreover, ratios of active heterotrophic biomass and autotrophic biomass (XH/XA) were obtained by performing respiration tests. By setting heterotroph/autotroph ratios obtained from sequencing analysis equal to XH/XA, a novel approach for kinetic model parameters estimation was developed. This work not only investigated microbial community of MBBR system, but also it provided an approach to make further use of molecular microbiology analysis results.
机译:微生物群落多样性决定了多级移动床生物膜反应器(MBBR)系统的每个腔室的功能。很少研究如何将微生物群落数据进一步用于废水处理过程的建模和优化。在这项研究中,建立了MBBR系统以调查每个功能室内生物膜的微生物群落多样性。通过高通量测序对来自不同MBBR室的生物膜微生物群落组成进行了定量。在第二有氧室中发现自养生物的比例更高(15.4%),而在第一有氧室中发现4.3%的自养生物。缺氧室内的自养生物可以忽略不计。此外,通过进行呼吸试验获得活性异养生物质和自养生物质的比值(XH / XA)。通过设置从测序分析获得的异养/自养比率等于XH / XA,开发了一种动力学模型参数估计的新方法。这项工作不仅研究了MBBR系统的微生物群落,而且为进一步利用分子微生物学分析结果提供了一种方法。

著录项

  • 来源
    《Journal of Environmental Management》 |2018年第15期|340-347|共8页
  • 作者单位

    Faculty of Science and Technology, Norwegian University of Life Sciences;

    Qingdao University of Technology, State and Local Joint Engineering Research Center of Urban Wastewater Treatment and Reclamation in China;

    Faculty of Science and Technology, Norwegian University of Life Sciences,Oslo Water and Sewage Works;

    Faculty of Science and Technology, Norwegian University of Life Sciences,Qingdao University of Technology, State and Local Joint Engineering Research Center of Urban Wastewater Treatment and Reclamation in China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Autotrophic biomass; Heterotrophic biomass; High-throughput sequencing; MBBR; Microbial community; Wastewater treatment;

    机译:自养生物量;异养生物量;高通量测序;MBBR;微生物群落;废水处理;

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