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Re-fermentation of washed spent solids from batch hydrogenogenic fermentation for additional production of biohydrogen from the organic fraction of municipal solid waste

机译:通过分批氢气发酵对发酵后的废固体进行再发酵,以从城市固体废物的有机部分额外生产生物氢

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摘要

In the first batch solid substrate anaerobic hydrogenogenic fermentation with intermittent venting (SSAHF-Ⅳ) of the organic fraction of municipal solid waste (0FMSW), a cumulative production of 16.6 mmol H_2/reactor was obtained. Releases of hydrogen partial pressure first by intermittent venting and afterward by flushing headspace of reactors with inert gas N_2 allowed for further hydrogen production in a second to fourth incubation cycle, with no new inoculum nor substrate nor inhibitor added. After the fourth cycle, no more H_2 could be harvested. Interestingly, accumulated hydrogen in 4 cycles was 100% higher than that produced in the first cycle alone. At the end of incubation, partial pressure of H_2 was near zero whereas high concentrations of organic acids and solvents remained in the spent solids. So, since approximate mass balances indicated that there was still a moderate amount of biodegradable matter in the spent solids we hypothesized that the organic metabolites imposed some kind of inhibition on further fermentation of digestates. Spent solids were washed to eliminate organic metabolites and they were used in a second SSAHF-Ⅳ. Two more cycles of H_2 production were obtained, with a cumulative production of ca. 2.4 mmol H_2/mini-reactor. As a conclusion, washing of spent solids of a previous SSAHF-Ⅳ allowed for an increase of hydrogen production by 15% in a second run of SSAHF-Ⅳ, leading to the validation of our hypothesis.
机译:在对城市固体废物(0FMSW)的有机部分进行间歇排气(SSAHF-Ⅳ)的第一批固体底物厌氧氢发酵中,获得的累计产量为16.6 mmol H_2 /反应器。首先通过间歇排气来释放氢分压,然后通过用惰性气体N_2冲洗反应器的顶部空间来释放氢分压,以便在第二至第四次培养循环中进一步产生氢,而无需添加新的接种物,底物或抑制剂。在第四个周期之后,无法再收获H_2。有趣的是,在4个循环中积累的氢比仅在第一个循环中产生的氢高100%。孵育结束时,H_2的分压接近零,而废固体中仍残留着高浓度的有机酸和溶剂。因此,由于近似的质量平衡表明,废固体中仍存在适量的可生物降解物质,因此我们假设有机代谢产物对消化物的进一步发酵产生某种抑制作用。洗涤用过的固体以消除有机代谢物,并将其用于第二个SSAHF-Ⅳ。获得了H_2产生的另外两个周期,累积产生量约为。 2.4 mmol H_2 /微型反应器。结论是,对以前的SSAHF-Ⅳ的废固体进行洗涤可以使第二次SSAHF-Ⅳ的产氢量增加15%,从而验证了我们的假设。

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  • 来源
    《Journal of Environmental Management》 |2012年第suppla期|p.S355-S359|共5页
  • 作者单位

    Environmental Biotechnology and Renewable Energies R&D Croup, Dept Biotechnology and Bioengineering, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico National, P.O. Box 14-740, Mexico D.F. 07000, Mexico;

    Central Analitica, Dept. Biotechnology and Bioengineering, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico National, Mexico D.F., Mexico;

    Environmental Biotechnology and Renewable Energies R&D Croup, Dept Biotechnology and Bioengineering, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico National, P.O. Box 14-740, Mexico D.F. 07000, Mexico;

    ESIOIE del IPN, Division de Ciencias Basicas, Campus Zacatenco, Mexico D.F., Mexico;

    Environmental Biotechnology and Renewable Energies R&D Croup, Dept Biotechnology and Bioengineering, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico National, P.O. Box 14-740, Mexico D.F. 07000, Mexico;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biohydrogen; organic fraction of municipal solid wastes; re-fermentation; solid substrate; spent solids; washing;

    机译:生物氢城市固体废物的有机部分;发酵固体基质废固体洗涤;

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