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Trace elements effect on hydrolytic stage towards biogas production of model lignocellulosic substrates

机译:微量元素影响模型木质纤维素底物沼气生产的水解阶段

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摘要

The effect and the response of several trace elements (TE) addition to the anaerobic degradation of key compounds of lignocellulosic biomass were evaluated. Lignin, cellulose and xylose were selected as principal compounds of lignocellulosic biomass. Lignin degradation was only improved by the addition of 1000 mg Fe/L, which allowed an improvement on the methane yield coefficient of 28% compared to control. SEM images from an abiotic assay showed that this effect is more likely related with a chemical effect induced by the Fe solution, instead of an enzymatic response. Pre-treatments focused on breaking the recalcitrant structure of the lignin could be more promising than TE addition for rich lignin-content substrates. Unlike to the response observed with lignin, cellulose showed a clear effect of the TE addition on methane production rate, indicating a higher preponderance of the enzymatic activity compared to the lignin biomethanization. Experiments with xylose resulted in a strong accumulation of volatile fatty acids. TE addition should be adapted to the substrate composition given the different response of each lignocellulosic compound to the different TE addition.
机译:评估了几种微量元素(TE)对木质纤维素生物质关键化合物厌氧降解的影响和响应。选择木质素,纤维素和木糖作为木质纤维素生物质的主要化合物。木质素的降解仅通过添加1000 mg Fe / L得以改善,与对照相比,甲烷的产率系数提高了28%。非生物测定的SEM图像表明,这种作用更可能与Fe溶液诱导的化学作用有关,而不是与酶促反应有关。对于富含木质素含量的底物,侧重于破坏木质素顽固结构的预处理可能比添加TE更有希望。与木质素观察到的响应不同,纤维素显示出TE添加对甲烷产生速率的明显影响,表明与木质素生物甲烷化相比,酶活性具有更高的优势。木糖的实验导致挥发性脂肪酸的大量积累。给定每种木质纤维素化合物对不同TE添加量的不同响应,TE添加量应适合底物组成。

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