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首页> 外文期刊>Journal of Environmental Management >Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes
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Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes

机译：使用荧光原位杂交（FISH）和微电极分析BioCube海绵培养基中的硝化细菌群落

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摘要

There is growing interest in the development of more cost-effective and retrofit technologies for the upgrade and expansion of existing wastewater treatment plants with extreme space constraints. A free-floating sponge media (BioCube) process, using a 24 L lab scale reactor, was operated to study the nitrification profiles and microbial community. The COD removal efficiencies were maintained, at an average of 95%, with the mixed liquor suspended solids (MLSS) inside the BioCube sponge media maintained at 12,688 mg/L. The nitrification removal efficiencies were between 92% and 100%, with an average value of 99%. From the results of microelectrode measurements, the ammonium ion concentration was found to rapidly decrease from the surface of the BioCube sponge media to a dep,th of 2 mm due to chemical reactions carried out by ammonia oxidizing bacteria (AOB) species. Multi-fluorescence in situ hybridization (FISH) has been used to investigate the spatial distributions of various microbial activities within reactors. Microbial communities were targeted using different oligonucleotide probes specific to AOB and nitrite oxidizing bacteria (NOB). There were a large number of AOB populations, but these were not uniformly distributed in the biofilm compared to the NOB populations.
机译：人们越来越关注开发更具成本效益的改造技术，以升级和扩展空间受限的现有废水处理厂。使用24 L实验室规模的反应器，进行自由漂浮的海绵介质（BioCube）工艺，以研究硝化特性和微生物群落。通过将BioCube海绵介质内部的混合液悬浮固体（MLSS）保持在12,688 mg / L，可将COD去除率平均保持在95％。硝化去除效率在92％至100％之间，平均值为99％。根据微电极测量的结果，由于氨氧化细菌（AOB）物种进行的化学反应，铵离子的浓度从BioCube海绵介质的表面迅速降低至2 mm的深度。多荧光原位杂交（FISH）已用于研究反应器内各种微生物活动的空间分布。使用对AOB和亚硝酸盐氧化细菌（NOB）具有特异性的不同寡核苷酸探针靶向微生物群落。有大量的AOB种群，但与NOB种群相比，它们在生物膜中分布不均匀。

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