• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Environmental Protection and Ecology >OXIDATION OF DIAZINON FOR THE SENSITIVE DETECTION BY CHOLINESTERASE-BASED BIO ANALYTICAL METHOD
【24h】

OXIDATION OF DIAZINON FOR THE SENSITIVE DETECTION BY CHOLINESTERASE-BASED BIO ANALYTICAL METHOD

机译：基于胆碱酯酶的生物分析方法对叠氮酮的氧化进行灵敏检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diaioxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholineste-rase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1×1(HM-1×10~(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 × 10~(-5) M, the detection limit after oxidation was below 1 × 10~(-5) M.
机译：使用酶髓过氧化物酶（MPO）作为氧化剂，研究了OPs的模型化合物diazinon向更有效的AChE抑制剂diaioxon的转化。目的是增强其抑制能力，以便开发用于环境检测的快速，有效和特异的基于乙酰胆碱酯酶（AChE）的生物分析方法。与样品的对照值相比，将1×1（HM-1×10〜（-7）M二嗪农酮与100 nM MPO一起孵育5分钟可将重氮x酮的形成将AChE抑制程度从80％提高到10％在未经处理的样品中含有相同的二嗪农浓度，构建了MPO孵育后通过AChE测定的二嗪农检测校准图，而氧化前二嗪农的最低检测限（抑制10％AChE）约为1×10〜（-5 ）M，氧化后的检出限低于1×10〜（-5）M.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号