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首页> 外文期刊>Journal of environmental science and health >Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro
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Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro

机译：多拉菌素对牛外周血淋巴细胞和卵丘细胞的细胞毒性和遗传毒性作用

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摘要

The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DOR were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, single cell gel electrophoresis assay (SCGE) and cytokinesis-block micronucleus cytome (CBMN Cyt) assay. Both cells were treated with three concentrations of DOR (20, 40, 60 ng mL(-1)) for 24 h. The results obtained from PL demonstrated that DOR was able to induce cytotoxic effect and DNA damage with all concentrations tested. Additionally, DOR increased micronuclei (MNi) frequency and nuclear buds (NBuds) with 20, 40, 60 ng mL(-1), and nucleoplasmic bridges (NPBs) only with 40 ng mL(-1). On the other hand, the three concentrations of DOR were not able to induce cytotoxic effect and DNA damage using SCGE in the bovine CC. Nevertheless, the two higher concentrations of DOR (20, 40 mu g mL(-1)) significantly increased the frequency of micronucleus formation in bovine CC. These results represent the first experimental evidence of genotoxic and cytotoxic effects exerted by DOR on bovine PL and CC.
机译：在体外对两种实验性体牛细胞测试了多拉菌素（DOR）的作用：外周淋巴细胞（PL）和积云细胞（CC）。使用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四唑（MTT）测定，单细胞凝胶电泳测定（SCGE）和胞质分裂阻滞剂评估DOR的细胞毒性和遗传毒性微核细胞因子（CBMN Cyt）分析。两个细胞都用三种浓度的DOR（20、40、60 ng mL（-1））处理24小时。从PL获得的结果表明，在所有测试浓度下，DOR都能诱导细胞毒性作用和DNA损伤。此外，DOR增加了20、40、60 ng mL（-1）的微核（MNi）频率和核芽（NBuds），以及仅添加40 ng mL（-1）的核质桥（NPB）。另一方面，在牛CC中使用SCGE，三种浓度的DOR均不能诱导细胞毒性作用和DNA损伤。尽管如此，两种较高浓度的DOR（20，40μg mL（-1））显着增加了牛CC中微核形成的频率。这些结果代表了DOR对牛PL和CC产生遗传毒性和细胞毒性作用的第一个实验证据。

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  • 来源
    《Journal of environmental science and health》 |2019年第3期|147-154|共8页
  • 作者

    Mateo Anchordoquy Juan; Patricio Anchordoquy Juan; Nikoloff Noelia; Gambaro Rocio; Padula Gisel; Seoane Analia; Furnus Cecilia;

  • 作者单位

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina|UNLP, Fac Ciencias Nat & Museo, Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

    UNLP, Fac Ciencias Vet, IGEVET, Inst Genet Vet Ing Fernando N Dulout UNLP CONICET, La Plata, Buenos Aires, Argentina;

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  • 正文语种 eng
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  • 关键词

    Bovine peripheral lymphocytes; bovine cumulus cells; doramectin; MTT; comet assay; CBMN Cyt assays;

    机译：牛外周淋巴细胞;牛卵丘细胞;多拉菌素;MTT;彗星分析;CBMN细胞分析;

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