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首页> 外文期刊>Journal of environmental science and health >Co-metabolic degradation of naphthalene and pyrene by acclimated strain and competitive inhibition kinetics
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Co-metabolic degradation of naphthalene and pyrene by acclimated strain and competitive inhibition kinetics

机译：驯化应变和竞争抑制动力学共同降解萘和pyr

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摘要

A dominant strain named Ochrobactrum sp. was isolated from soils contaminated with coal tar. The batch experiments were carried out to study the co-metabolic degradation of pyrene by Ochrobactrum MB-2 with naphthalene as the main substrate and the effects of several significant parameters such as naphthalene concentration, pH and temperature on removal efficiency were explored. The results showed that Ochrobactrum MB-2 effectively degraded naphthalene and that the addition of naphthalene favored the degradation of pyrene. The maximum elimination efficiency of naphthalene (10 mg L-1) and pyrene (1 mg L-1) was achieved at pH 7 and 25 degrees C, and the corresponding values were 99 and 41%, respectively. A competitive inhibition model based on the Michaelis-Menten equation was used to characterize the inhibitory effect of pyrene on naphthalene degradation. The values of the half-saturation coefficient for naphthalene (K-S) and dissociation constant of enzyme-inhibitor complex (K-C) were determined to be 4.93 and 1.38 mg L-1, respectively.
机译：显性菌株名为Ochrobactrum sp。从煤焦油污染的土壤中分离出来。进行了批处理实验，研究了以萘为主要底物的ch牙菌MB-2对-的共代谢降解，并探讨了萘浓度，pH和温度等几个重要参数对去除效率的影响。结果表明O菜MB-2能有效降解萘，而萘的加入有利于the的降解。在pH值为7和25℃时，萘（10 mg L-1）和pyr（1 mg L-1）的最大消除效率分别为99％和41％。使用基于Michaelis-Menten方程的竞争性抑制模型来表征of对萘降解的抑制作用。萘的半饱和系数（K-S）和酶抑制剂复合物（K-C）的解离常数分别确定为4.93和1.38 mg L-1。

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  • 来源
    《Journal of environmental science and health》 |2019年第6期|505-513|共9页
  • 作者

    Wang Zhen; Wang Wenjing; Li Yalong; Yang Qi;

  • 作者单位

    China Univ Geosci, Sch Water Resources & Environm, Beijing, Peoples R China|China Univ Geosci, Beijing Key Lab Water Resource & Environm Engn, Beijing 100083, Peoples R China;

    China Univ Geosci, Sch Water Resources & Environm, Beijing, Peoples R China|China Univ Geosci, Beijing Key Lab Water Resource & Environm Engn, Beijing 100083, Peoples R China;

    China Univ Geosci, Sch Water Resources & Environm, Beijing, Peoples R China|China Univ Geosci, Beijing Key Lab Water Resource & Environm Engn, Beijing 100083, Peoples R China;

    China Univ Geosci, Sch Water Resources & Environm, Beijing, Peoples R China|China Univ Geosci, Beijing Key Lab Water Resource & Environm Engn, Beijing 100083, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Naphthalene; pyrene; biodegradation; co-metabolism; competitive inhibition;

    机译：萘;py;生物降解;共代谢;竞争抑制;

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