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首页> 外文期刊>Journal of environmental science and health >Degradation of chlorpyrifos by an endophytic bacterium of the Sphingomonas genus (strain HJY) isolated from Chinese chives {Allium tuberosum)
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Degradation of chlorpyrifos by an endophytic bacterium of the Sphingomonas genus (strain HJY) isolated from Chinese chives {Allium tuberosum)

机译：从韭菜（葱属）分离的鞘氨醇单胞菌属（HJY菌株）的内生细菌降解毒死rif

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摘要

The degradation of chlorpyrifos (CP) by an endophytic bacterial strain (HJY) isolated from Chinese chives (Allium tuberosum Rottl. ex Spreng) was investigated. Strain HJY was identified as Sphingomonas sp. based on morphological, physiological, and biochemical tests and a 16S rDNA sequence analysis. Approximately 96% of 20 mg L~(-1) CP was degraded by strain HJY over 15 days in liquid minimal salts medium (MSM). The CP degradation rate could also be increased by glucose supplementation. The optimal conditions for the removal of 20 mg L~(-1) CP by strain HJY in MSM were 2% inoculum density, pH 6.0, and 30-35℃. The CP degradation rate constant and half-life were 0.2136 ± 0.0063 d~(-1) and 3.2451 ± 0.0975 d, respectively, under these conditions, but were raised to 0.7961 ± 0.1925 d~(-1) and 0.8707 ± 0.3079 d with 1% glucose supplementation. The detection of metabolic products and screening for degrading genes indicated that O,O-diethyl 0-3,5,6-trichioropyridinol was the major degradation product from CP, while it was likely that some functional genes were undetected and the mechanism responsible for CP degradation by strain HJY remained unknown. Strain HJY is potentially useful for the reduction of CP residues in Chinese chives and may be used for the in situ phytoremediation of CP.
机译：研究了从韭菜（Allium tuberosum Rottl。ex Spreng）分离的内生细菌菌株（HJY）对毒死rif（CP）的降解作用。 HJY菌株被鉴定为鞘氨醇单胞菌。基于形态，生理和生化测试以及16S rDNA序列分析。 HJY菌株在液态基本食盐培养基（MSM）中历时15天降解了约96％的L〜（-1）CP。还可以通过补充葡萄糖来提高CP降解率。 HJY菌株在MSM中去除20 mg L〜（-1）CP的最佳条件是接种密度2％，pH 6.0和30-35℃。在这些条件下，CP降解速率常数和半衰期分别为0.2136±0.0063 d〜（-1）和3.2451±0.0975 d，但在90℃时分别提高到0.7961±0.1925 d〜（-1）和0.8707±0.3079 d。 1％葡萄糖补充。代谢产物的检测和降解基因的筛选表明，O，O-二乙基0-3,5,6-三氯吡啶酚是CP的主要降解产物，而某些功能基因很可能未被检测到且可能是CP的原因。 HJY菌株的降解仍然未知。 HJY菌株可能对减少韭菜中CP残留物有用，并可用于CP的原位植物修复。

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  • 来源
    《Journal of environmental science and health》 |2017年第12期|736-744|共9页
  • 作者

    Fayun Feng; Yisong Li; Jing Ge; Jinjin Chen; Wayne Jiang; Shuang He; Xianjing Liu; Xiangyang Yu;

  • 作者单位

    Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China,lnstitute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China;

    Department of Plant Protection, Agricultural College, Shihezi University, Xinjiang, China;

    Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China,lnstitute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China;

    Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China;

    Department of Entomology, Michigan State University, East Lansing, Michigan, USA;

    lnstitute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China,Department of Plant Protection, Agricultural College, Shihezi University, Xinjiang, China;

    lnstitute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China;

    Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China,lnstitute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China;

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  • 正文语种 eng
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  • 关键词

    Endophytic bacterium; chlorpyrifos; degradation; Sphingomonas sp;

    机译：内生细菌;毒死rif;降解;鞘氨醇单胞菌;

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