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Isolation and identification of 3-bromocarbazole-degrading bacteria

机译:降解3-溴咔唑的细菌的分离与鉴定

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摘要

In this study, a bacterial strain, CH-1, capable of degrading 3-bromocarbazole (3-BCZ) was isolated from a polluted soil. Based on its physio-biochemical characteristics and 16S rRNA genes, strain CH-1 was identified as a Stenotrophomonas sp. Strain CH-1 was able to degrade 70% of 50 mg/L 3-BCZ within 8 d at pH 7.0 and 30℃ in mineral salt medium (MSM). During the process, the main intermediate metabolite was identified as (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2, 4-dienoic by gas (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2,4-dienoic via gas chromatograph-mass spectrometry (GC-MS) analysis. The metabolite disappeared after 14 d, suggesting that the metabolite can also be degraded by strain CH-1. 3-BCZ is a new persistent organic pollutant. This is the first report of the biodegradation of 3-BCZ. The results indicated that strain CH-1 may be a promising bacterial candidate for the bioremediation of environments polluted with polyhalogenated carbazoles (PHCs).
机译:在这项研究中,从污染的土壤中分离出了能够降解3-溴咔唑(3-BCZ)的细菌菌株CH-1。根据其生理生化特性和16S rRNA基因,菌株CH-1被鉴定为嗜麦芽单胞菌。 CH-1菌株能够在pH 7.0和30℃的矿物盐培养基(MSM)中在8天内降解70%的50 mg / L 3-BCZ。在该过程中,主要中间代谢物通过气体(2E,4Z)-被鉴定为(2E,4Z)-6-(2-氨基-5-溴苯基)-2-羟基-6-氧六-2,4-二烯酸。通过气相色谱-质谱(GC-MS)分析获得6-(2-氨基-5-溴苯基)-2-羟基-6-氧己-2,4-二烯酸。代谢产物在14 d后消失,表明该代谢物也可被菌株CH-1降解。 3-BCZ是一种新型的持久性有机污染物。这是3-BCZ生物降解的首次报道。结果表明,菌株CH-1可能是对多卤代咔唑(PHC)污染的环境进行生物修复的有前途的细菌候选物。

著录项

  • 来源
    《Journal of environmental science and health》 |2017年第12期|796-801|共6页
  • 作者单位

    Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, People's Republic of China;

    Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, People's Republic of China;

    Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, People's Republic of China;

    Environmental Science Research Institute of Taizhou City, Taizhou, China;

    Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    3-Bromocarbazole; new persistent organic pollutant; biodegradation; Stenotrophomonas sp.; intermediate metabolites;

    机译:3-溴咔唑;新的持久性有机污染物;生物降解嗜麦芽单胞菌中间代谢物;

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