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首页> 外文期刊>Journal of environmental science and health >Fatty acids composition of Caenorhabditis elegans using accurate mass GCMS-QTOF
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Fatty acids composition of Caenorhabditis elegans using accurate mass GCMS-QTOF

机译:使用精确质量数GCMS-QTOF进行秀丽隐杆线虫的脂肪酸组成

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The free living nematode Caenorhabditis elegans is a proven model organism for lipid metabolism research. Total lipids of C. elegans were extracted using chloroform and methanol in 2:1 ratio (v/v). Fatty acids composition of the extracted total lipids was converted to their corresponding fatty acids methyl esters (FAMEs) and analyzed by gas chromatography/accurate mass quadrupole time of flight mass spectrometry using both electron ionization and chemical ionization techniques. Twenty-eight fatty acids consisting of 12 to 22 carbon atoms were identified, 65% of them were unsaturated. Fatty acids containing 12 to17 carbons were mostly saturated with stearic acid (18:0) as the major constituent. Several branched-chain fatty acids were identified. Methyl-14-methylhexadecanoate (iso- 17:0) was the major identified branched fatty acid. This is the first report to detect the intact molecular parent ions of the identified fatty acids in C. elegans using chemical ionization compared to electron ionization which produced fragmentations of the FAMEs.
机译:自由生活的线虫秀丽隐杆线虫是用于脂质代谢研究的成熟模型生物。使用氯仿和甲醇以2:1的比例(v / v)提取线虫的总脂质。将提取的总脂质的脂肪酸组成转化为相应的脂肪酸甲酯(FAME),并使用电子电离和化学电离技术,通过气相色谱/精确质量四极飞行时间质谱法进行分析。鉴定出由12至22个碳原子组成的28种脂肪酸,其中65%为不饱和脂肪酸。含12至17个碳的脂肪酸主要以硬脂酸(18:0)为主要成分饱和。鉴定了几种支链脂肪酸。鉴定出的主要支链脂肪酸是14-甲基十六烷酸甲酯(iso-17:0)。这是第一个报告,使用化学电离与电子电离产生线虫的碎片,检测线虫中鉴定出的脂肪酸的完整分子母体离子。

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