...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental science and health >Production of the broad specific monoclonal antibody against sarafloxacin for rapid immunoscreening of 12 fluoroquinolones in meat
【24h】

Production of the broad specific monoclonal antibody against sarafloxacin for rapid immunoscreening of 12 fluoroquinolones in meat

机译：产生广泛的抗塞拉沙星单克隆抗体，用于快速免疫筛选肉类中的12种氟喹诺酮

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of this study was to produce a generic antibody for immunoassay of fluoroquinolone drugs in meat. Two novel haptens of sarafloxacin were synthesized that were used to produce the monoclonal antibodies. The obtained monoclonal antibodies simultaneously recognized 12 fluoroquinolone drugs (sarafloxacin, diflocaxin, marbofloxacin, ofloxacin, ciprofloxacin, enrofloxacin, nor-floxacin, pefloxacin, lomefloxacin, amifloxacin, enofloxacin and danofloxacin). After evaluation of different coating antigen/antibody combinations, a heterologous competitive indirect enzyme linked immunosorbent assay (ELISA) was developed to determine the 12 drugs. The crossreactivities to these analytes were in the range of 18％-113％ and the limits of detection were in the range of 0.8-6.5 ng/mL depending on the compound. Eight fluoroquinolones licensed as veterinary drugs in China were fortified into blank chicken for ELISA analysis. The recoveries were in the range of 67.6％-94.6％ with coefficients of variation lower than 12.4％. Therefore, this method could be used as a screen tool for routine monitoring of the residues of these fluoroquinolone drugs in animal derived foods.
机译：这项研究的目的是生产一种用于肉类中氟喹诺酮类药物免疫分析的通用抗体。合成了两种新的赛拉沙星半抗原，用于生产单克隆抗体。所获得的单克隆抗体同时识别12种氟喹诺酮药物（沙洛沙星，地氟卡辛，马波沙星，氧氟沙星，环丙沙星，恩诺沙星，去甲沙星，培氟沙星，洛美沙星，阿米沙星，依诺沙星和达诺沙星）。在评估了不同的包被抗原/抗体组合后，开发了异源竞争性间接酶联免疫吸附测定（ELISA）来确定12种药物。对这些分析物的交叉反应性在18％-113％范围内，检测限在0.8-6.5 ng / mL范围内，具体取决于化合物。将八种在中国获准用作兽药的氟喹诺酮类药物制成空白鸡进行ELISA分析。回收率在67.6％-94.6％的范围内，变异系数小于12.4％。因此，该方法可用作常规监测动物衍生食品中这些氟喹诺酮药物残留的筛选工具。

著录项

  • 来源
    《Journal of environmental science and health》 |2013年第3期|139-146|共8页
  • 作者

    YAN Z. LIU; GUO X. ZHAO; PING WANG; JING LIU; HUI C. ZHANG; JIAN P. WANG;

  • 作者单位

    College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China,Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China;

    College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China,Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China;

    Hebei Institute of Veterinary Drug Control, Shijiazhuang Hebei, China;

    College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China,Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China;

    College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China,Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China;

    College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China,Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Fluoroquinolones; sarafloxacin; monoclonal antibody; heterologous ELISA; chicken;

    机译：氟喹诺酮类;沙拉沙星单克隆抗体;异源ELISA鸡;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号