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首页> 外文期刊>Journal of environmental science and health >Increased virulence of transgenic Trichoderma koningi strains to the Asian corn borer larvae by overexpressing heterologous chit42 gene with chitin-binding domains
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Increased virulence of transgenic Trichoderma koningi strains to the Asian corn borer larvae by overexpressing heterologous chit42 gene with chitin-binding domains

机译:通过过表达具有几丁质结合域的异源chit42基因,提高转基因木霉菌对亚洲玉米bore幼虫的毒力

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摘要

The chit42 gene cloned from Metarhizium anisopliae lacks chitin-binding domain (chBD), which plays important roles in binding insoluble chitin. Five kinds of hybrid chitinase Trichoderma transformants were constructed in this study, where the chit42 gene was fused to chBDs derived from plant, bacterial, and insect sources. The transformant Mc4 harboring chBDs from bitter melon (Momordica charantia) displayed the highest chitinase activity among all chBDs. The chitinase activities of Mc4, chit42 Trichoderma transformant Mchit3, and wild-type strain T30 were 44.94, 32.48, and 12.38 U/mL, respectively. The mortality rate of corn borer larvae in Mc4 fermentation liquid treatment increased by 10% and 30% compared with Mchit3 and T30, respectively. The midgut microvilli and goblet cell microvilli of the corn borer larvae exhibited distinct pathological changes after 48 h of feeding in Mc4 treatment. Mc4 also exhibited the strongest antifungal activity against Fnsarium verticillioides and Rhizoctonia solani.
机译:克隆自金属异化菌的chit42基因缺少几丁质结合结构域(chBD),其在结合不溶性几丁质中起重要作用。在这项研究中,构建了五种杂化几丁质酶木霉属转化子,其中chit42基因与植物,细菌和昆虫来源的chBD融合。带有苦瓜(Momordica charantia)chBDs的转化体Mc4在所有chBDs中都表现出最高的几丁质酶活性。 Mc4,chit42木霉转化体Mchit3和野生型菌株T30的几丁质酶活性分别为44.94、32.48和12.38 U / mL。与Mchit3和T30相比,Mc4发酵液处理中的玉米bore幼虫死亡率分别提高了10%和30%。饲喂Mc4处理的玉米treatment幼虫的中肠微绒毛和杯状细胞微绒毛表现出明显的病理变化。 Mc4还表现出最强的抗Fnsarium v​​erticillioides和立枯丝核菌的抗真菌活性。

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