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首页> 外文期刊>Journal of environmental science and health >Optimization of methyl parathion biodegradation and detoxification by cells in suspension or immobilized on tezontle expressing the opd gene
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Optimization of methyl parathion biodegradation and detoxification by cells in suspension or immobilized on tezontle expressing the opd gene

机译：悬浮或固定在表达opd基因的tezontle上的细胞对甲基对硫磷的生物降解和解毒作用的优化

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The goal of this study was to optimize methyl parathion (O, O-dimethyl-O-4-p-nitrophenylphosphorothioate) degradation using a strain of Escherichia toll DH5α expressing the opd gene. Our results indicate that this strain had lower enzymatic activity compared to the Flavobacterium sp. ATCC 27551 strain from which the opd gene was derived. Both strains were assessed for their ability to degrade methyl parathion (MP) in a mineral salt medium with or without the addition of glucose either as suspended cells or immobilized on tezontle, a volcanic rock. MP was degraded by both strains with similar efficiencies, but immobilized cells degraded MP more efficiently than cells in suspension. However, the viability of E. coli cells was much higher than that of the Flavobacterium sp. We confirmed the decrease in toxicity from the treated effluents through acetylcholinesterase activity tests, indicating the potential of this method for the treatment of solutions containing MP.
机译：这项研究的目的是使用表达opd基因的大肠杆菌收费DH5α菌株，优化甲基对硫磷（O，O-二甲基-O-4-对硝基苯基硫代磷酸酯）的降解。我们的结果表明，与黄杆菌属菌种相比，该菌株具有较低的酶促活性。产生opd基因的ATCC 27551菌株。评估了两种菌株在矿物盐培养基中降解甲基对硫磷（MP）的能力，添加或不添加葡萄糖作为悬浮细胞或固定在火山岩tezontle上。两种菌株均以相似的效率降解了MP，但是固定化细胞比悬浮细胞降解MP的效率更高。但是，大肠杆菌细胞的活力远高于黄杆菌属。我们通过乙酰胆碱酯酶活性测试证实了处理过的废水的毒性降低，表明该方法对处理含MP溶液的潜力。

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