...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental science and health >Acridine orange as an alternative to optical density to study, growth kinetics of Lactobacillus bulgaricus ATCC 7517
【24h】

Acridine orange as an alternative to optical density to study, growth kinetics of Lactobacillus bulgaricus ATCC 7517

机译：cr啶橙可替代光密度来研究保加利亚乳杆菌ATCC 7517的生长动力学

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study we assessed the use of acridine orange as an alternative to optical density to quantify the growth of Lactobacillus bulgaricus ATCC 7517. The growth of bacteria in Lactobacillus de Man Rogosa Sharpe (MRS) medium was measured by both acridine orange (AO) and optical density (OD) measurements for 24 h. The relationship between both methods was compared via correlation analysis. The doubling time of bacteria based on the values of OD_(600) and AO obtained during 24 h growth were also calculated. The result shows strong correlation of cell growth between OD_(600) and AO during the first 10 hours of growth, but the correlation was less strong when analyzing the data from 0 to 24 hours. Growth rates, generation time and lag time were also similar. This study indicates that AO could be used in place of OD to prepare growth curves of Lactobacillus bulgaricus during the exponential phase of growth, and to compare growth rates, generation times or lag times.
机译：在这项研究中，我们评估了使用cr啶橙替代光密度来量化保加利亚乳杆菌ATCC 7517的生长。通过Man啶橙（AO）和cr啶橙测量了乳酸杆菌在人Rogosa Sharpe（MRS）培养基中的生长。光密度（OD）测量24小时。通过相关分析比较了这两种方法之间的关系。还基于在24 h生长期间获得的OD_（600）和AO值计算了细菌的倍增时间。结果表明，在生长的前10小时内，OD_（600）和AO之间的细胞生长具有很强的相关性，但在分析0至24小时的数据时，相关性不那么强。增长率，产生时间和滞后时间也相似。这项研究表明，可以使用AO代替OD来制作保加利亚乳杆菌在指数生长期的生长曲线，并比较其生长速度，发生时间或滞后时间。

著录项

  • 来源
    《Journal of environmental science and health》 |2013年第6期|512-515|共4页
  • 作者

    DOLAR PAK; OK KYUNG KOO; ROBERT S. STORY; CORLISS A. OBRYAN; PHILIP G. CRANDALL; SUN-OK LEE; STEVEN C. RICKE;

  • 作者单位

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

    Center for Food Safety, Dept. of Food Science, University of Arkansas, Fayetteville, Arkansas, USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lactobacillus; acridine orange; growth kinetics;

    机译：乳杆菌;cr啶橙生长动力学;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号