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首页> 外文期刊>Journal of environmental science and health >The integration of in vivo and in vitro metabolism assays to investigate the stereoselective behaviors of benalaxyl in rainbow trout (Oncorhynchus my kiss)
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The integration of in vivo and in vitro metabolism assays to investigate the stereoselective behaviors of benalaxyl in rainbow trout (Oncorhynchus my kiss)

机译：体内和体外代谢检测方法的整合，用于研究虹鳟中苯那西尔的立体选择性行为（Oncorhynchus my kiss）

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摘要

The bioconcentration and elimination of racemic benalaxyl (BX) in trout liver microsomes and in juvenile rainbow trout (Oncorhynchus mykiss) were investigated to determine whether the fish can bioconcentrate and degrade this fungicide enantioselectively. Both enantiomers of BX were extracted with organic solvents and evaluated using high-performance liquid chromatography. In the microsomes, BX degradation followed first-order kinetics, and the S-(+) enantiomer of BX was eliminated twice as rapidly as the R-(-) enantiomer, resulting in residues enriched for R-(-)-BX. In vivo experiment, chiral analysis showed an obvious selective bioconcentration of BX based on statistically altered enantiomer fractions (EFs) in the fish compared with the values in the water. The R-(-)-BX was initially preferentially bioconcentrated by rainbow trout and then dissipated more slowly than its antipode. The mean half-lives for individual enantiomers were calculated as 31.6 h for R-(-)-BX and 20.3 h for the S-(+)-form. The results of the study showed that the degradation of BX enantiomers was stereoselective in rainbow trout.
机译：对鳟鱼肝微粒体和少年虹鳟鱼（Oncorhynchus mykiss）中的外消旋苯那西林（BX）的生物浓缩和消除进行了研究，以确定该鱼是否可以生物浓缩和对映选择性降解这种杀菌剂。 BX的两种对映体均用有机溶剂萃取，并使用高效液相色谱法进行评估。在微粒体中，BX降解遵循一级动力学，BX的S-（+）对映异构体的清除速度是R-（-）对映体的两倍，从而导致残基富含R-（-）-BX。在体内实验中，手性分析表明，与水中的对映体分数（EFs）相比，鱼体内的BX具有选择性的生物富集度，这是通过统计学上改变的对映体分数（EFs）决定的。 R-（-）-BX最初优选通过虹鳟鱼进行生物浓缩，然后耗散比其对映体慢。对于R-（-）-BX，单个对映体的平均半衰期为31.6小时，对于S-（+）-形式，为20.3小时。研究结果表明，虹鳟鱼中BX对映体的降解具有立体选择性。

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  • 来源
    《Journal of environmental science and health》 |2013年第9期|693-699|共7页
  • 作者

    XU GU; TING YAO;

  • 作者单位

    Key Laboratory of Feed Biotechnology, Ministry of Agriculture of China, Beijing, China ,Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China,Feed Research Institute, Chinese Academy of Agricultural Sciences, Zhongguancun South Street 12, Beijing 100081, P.R. China;

    Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China;

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  • 正文语种 eng
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  • 关键词

    Benalaxyl; rainbow trout; stereoselectivity; bioconcentration; degradation;

    机译：贝那克;虹鳟鱼;立体选择性生物浓缩降解;

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