...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental science and health >Evaluation of bendiocarb cytotoxicity in mammalian and insect cell cultures
【24h】

Evaluation of bendiocarb cytotoxicity in mammalian and insect cell cultures

机译：评估苯达威在哺乳动物和昆虫细胞培养物中的细胞毒性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is an increasing need for rapid and easily interpreted in vitro assays to screen for possible cytotoxicity of pesticides. The objective of this study was to investigate the effect of the carbamate insecticide bendiocarb on mammalian and insect cell cultures. The cytotoxicity of this insecticide was evaluated by cell proliferation and cellular damage was assessed by evaluation of the cytopathic effect and lactate dehydrogenase (LDH) leakage. Cells of insect origin (Sf21) were the most sensitive to bendiocarb with significant (P < 0.01) suppression of their proliferative activity ranging from 10~(-1)-10~(-5) M. However, significant suppression of proliferative activity was also recorded in rat liver cells (WBF344; 10~(-1)-10~(-3) M; P < 0.01-0.05) and rabbit kidney cells (RK13; 10~(-1) M; P < 0.01). In contrast with the proliferation activity of cells, a cytopathic effect based on cellular damage and LDH leakage into the medium was observed only at the highest concentration (10~(-1) M) in RK 13 and WBF344 cells, but not in the Sf21 insect cell line. Our results indicate that bendiocarb exposure caused a cell-type dependent decrease in cell proliferation; however, cell damage and LDH leakage into the medium were not present or were strongly limited, dependent on the cell phenotype. Cell proliferation was shown as a sensitive indicator for evaluation of the cytotoxic effect of bendiocarb in vitro; on the other hand, microscopic signs of cellular damage and LDH leakage were insufficient in vitro markers.
机译：越来越需要快速且容易解释的体外测定法来筛选农药的可能细胞毒性。这项研究的目的是研究氨基甲酸酯杀虫剂苯达威对哺乳动物和昆虫细胞培养的影响。通过细胞增殖评估该杀虫剂的细胞毒性，并通过评估细胞病变作用和乳酸脱氢酶（LDH）泄漏评估细胞损伤。昆虫起源的细胞（Sf21）对苯达威威最敏感，其增殖活性受到显着（P <0.01）抑制，范围从10〜（-1）-10〜（-5）M。但是，对增殖活性的显着抑制是在大鼠肝细胞（WBF344; 10〜（-1）-10〜（-3）M; P <0.01-0.05）和兔肾细胞（RK13; 10〜（-1）M; P <0.01）中也有记录。与细胞的增殖活性相反，仅在RK 13和WBF344细胞中以最高浓度（10〜（-1）M）观察到基于细胞损伤和LDH渗入培养基的细胞病变作用，而在Sf21中则没有昆虫细胞系。我们的结果表明，苯达威威暴露导致细胞增殖的细胞类型依赖性减少；然而，取决于细胞表型，细胞损伤和LDH向培养基中的泄漏不存在或受到严格限制。细胞增殖被证明是评估体外灭草威细胞毒性作用的敏感指标。另一方面，细胞损伤和LDH渗漏的微观迹象不足以作为体外标记。

著录项

  • 来源
    《Journal of environmental science and health》 |2012年第6期|p.538-543|共6页
  • 作者

    JANA POLLAKOVA; NATALIA KOVALKOVICOVA; TOMAS CSANK; JURAJ PISTL; ALICA KOCISOVA; JAROSLAV LEGATH;

  • 作者单位

    Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia;

    Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia;

    Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia;

    Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia;

    Department of Epizootology and Parasitology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia;

    Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    insecticide; bendiocarb; cell cultures; cell proliferation; cytotoxic effect; LDH;

    机译：杀虫剂灭草威细胞培养;细胞增殖;细胞毒性作用;乳酸脱氢酶;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号