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首页> 外文期刊>Journal of environmental science and health >Spectral characterization and chiral interactions of plant microsomal cytochrome P450 with metolachlor and herbicide safeners
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Spectral characterization and chiral interactions of plant microsomal cytochrome P450 with metolachlor and herbicide safeners

机译：植物微粒体细胞色素P450与异丙甲草胺和除草剂安全剂的光谱表征和手性相互作用

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The content and spectral characteristics of cytochrome P450 (Cyt P450) and cytochrome b_5 (Cyt bs) extracted from shoots of etiolated maize and rice seedlings were studied by using ultraviolet (UV) difference spectrophotometry. The results showed that fenclorim, rac-metolachlor and S-metolachlor may induce the same P450 isoenzyme with Xmax at 453 nm, while naphthalic anhydride (NA) induced another one with λ_(max) at 447 nm. The microsomal Cyt P450 and Cyt b_5 content of maize seedlings was higher than that of rice, and the Cyt b_5 content was higher than that of Cyt P450. Maize and rice microsomal Cyt P450 and Cyt b_5 were induced at different levels by the four chemicals, with the order as follows: NA > fenclorim > rac-metolachlor > S-metolachlor with p<0.05. When induced by NA, fenclorim, rac-metolachlor and S-metolachlor, the maize Cyt P450 content was, respectively, 5.63-, 3.30-, 3.02- and 2.48-fold that of the control, the rice Cyt P450 content was 8.54-, 2.20-, 1.91- and 1.33-fold that of the control, the maize Cyt b_5 content was 9.89-, 5.49-, 4.69- and 3.40-fold that of the control, and the rice Cyt b_5 content was 7.76-, 4.56-, 2.60- and 1.82-fold that of the control. An enantio-difference existed when rac- and S-metolachlor combined with plant Cyt P450. The interaction of microsomal Cyt P450 with S-metolachlor is higher than that with rac-metolachlor, which may be one of the reasons why S-metolachlor is superior at killing weeds compared with rac-metolachlor. These results will help to develop an understanding of the tolerance for and selectivity of rac-and S-metolachlor.
机译：采用紫外（UV）差光分光光度法研究了从黄化玉米和水稻幼苗的嫩芽中提取的细胞色素P450（Cyt P450）和细胞色素b_5（Cyt bs）的含量和光谱特性。结果表明，苯丙草胺，rac-异丙甲草胺和S-异丙甲草胺可以诱导相同的P450同工酶，其Xmax在453 nm，而萘酐（NA）诱导另一种P450同工酶的λ_（max）在447 nm。玉米幼苗的微粒体Cyt P450和Cyt b_5含量高于水稻，Cyt b_5含量高于Cyt P450。四种化学物质分别以不同的水平诱导了玉米和水稻微粒体Cyt P450和Cyt b_5的产生，顺序为：NA>苯丙草胺> rac-异丙甲草胺> S-异丙甲草胺，p <0.05。当由NA，苯甲草胺，rac-metolachlor和S-metolachlor诱导时，玉米Cyt P450的含量分别是对照的5.63-，3.30-，3.02-和2.48倍，水稻Cyt P450的含量为8.54-，玉米Cyt b_5含量是对照的2.20-，1.91-和1.33倍，玉米Cyt b_5含量是对照的9.89-，5.49-，4.69-和3.40倍，水稻Cyt b_5含量是对照的7.76-，4.56-，分别是对照的2.60倍和1.82倍。当rac-和S-异丙甲草胺与植物Cyt P450结合时，存在对映体差异。微粒体Cyt P450与S-异丙甲草胺的相互作用高于与rac-异丙甲草胺的相互作用，这可能是S-异丙甲草胺在杀灭杂草方面优于rac-甲草胺的原因之一。这些结果将有助于加深对rac-和S-异丙甲草胺的耐受性和选择性的理解。

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