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首页> 外文期刊>Journal of environmental science and health >Ex situ and in situ biodegradation of lindane by Azotobacter chroococcum
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Ex situ and in situ biodegradation of lindane by Azotobacter chroococcum

机译：绿脓杆菌对林丹的异位和原位生物降解

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摘要

This study was undertaken to investigate the potential of Azotobacter chroococcum for degrading lindane. Ten cultures were screened for lindane degradation by a chloride estimation method and the best A. chroococcum culture JL 102 was selected for further studies. This strain was subjected to a lindane-tolerance experiment and based on the results obtained, 10 and 100 ppm of lindane were selected to study the potential of the A. chroococcum strain for ex situ and in situ biodegradation of lindane. The organism was grown in 2 different media viz. Jensen's broth and soil extract broth and ex situ lindane degradation was studied for a period of 6 days. Maximum degradation of lindane was recorded at 10 ppm concentration. The degradation was higher in Jensen's medium, compared to the soil extract broth. A pot culture experiment was conducted using both sterile and non-sterile soils supplemented with 10 ppm lindane to study in situ degradation potential of this strain for a period of 8 weeks. In both the conditions (sterile and non-sterile), the organism exhibited increased degradation over the days with maximum degradation observed on the 8th week of incubation. It could degrade most of the applied lindane by the end of the study period.
机译：进行这项研究是为了研究嗜蓝固氮菌降解林丹的潜力。通过氯化物估算方法筛选了十种培养物中林丹的降解情况，并选择了最佳的嗜酸曲霉培养物JL 102进行进一步研究。对该菌株进行林丹耐受性实验，并基于获得的结果，选择10 ppm和100 ppm的林丹来研究A. chococcum菌株对林丹的非原位和原位生物降解的潜力。该生物在两种不同的培养基中生长。研究了詹森氏肉汤和土壤提取物肉汤以及异地林丹的降解情况，为期6天。在10 ppm浓度下记录到林丹的最大降解。与土壤提取物肉汤相比，在詹森培养基中的降解程度更高。使用添加了10 ppm林丹的无菌和非无菌土壤进行盆栽实验，以研究该菌株的原位降解潜力，为期8周。在两种条件下（无菌和非无菌），生物体在几天中的降解都增加，在孵育的第8周观察到最大程度的降解。在研究期结束时，它可以降解大多数应用的林丹。

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