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首页> 外文期刊>Journal of environmental science and health >The effect of pesticides and metals on acetylcholinesterase (AChE) in various tissues of blue mussel (Mytilus trossulus L.) in short-term in vivo exposures at different temperatures
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The effect of pesticides and metals on acetylcholinesterase (AChE) in various tissues of blue mussel (Mytilus trossulus L.) in short-term in vivo exposures at different temperatures

机译:农药和金属在不同温度下短期体内暴露对蓝贻贝(Mytilus trossulus L.)各种组织中乙酰胆碱酯酶(AChE)的影响

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摘要

The purpose of the study was to assess the impact of short-term exposure to selected toxicants as well as metal accumulation upon acetylcholinesterase (AChE) in the blue mussel, Mytilus trossulus L., in laboratory in vivo experiments. Mussels were exposed for up to 48 hours to a mixture of copper (Cu~(2+), 400 μg L~(-1)) and cadmium (Cd~(2+), 200 μg L~(-1)), to dichlorvos (DDVP, 100 μg L~(-1)), and to carbaryl (100 μg L~(-1)) at two temperatures: 5℃ and 20℃. Samples were collected after 0, 6, 12, 24, and 48 hours of exposure, and AChE activity and metal concentration (where applicable) were analysed in gills, digestive gland, mantle+muscles, and the whole soft tissue. Very strong AChE inhibition was observed in response to the dichlorvos treatment, mainly in gills. Carbaryl and the metals caused a short-term inhibition effect. Considerable differences in AChE activity between the two temperatures were noticed. In particular, the metals were accumulated much faster at 20℃ than at 5℃, especially in gills. No correlation between AChE activity and metal concentration was found. Gills turned out to be the optimal tissue for AChE activity analysis in short-term studies.
机译:该研究的目的是在实验室体内实验中评估短期暴露于选定的有毒物质以及金属积累对蓝贻贝Mytrous trossulus L.中的乙酰胆碱酯酶(AChE)的影响。贻贝暴露于铜(Cu〜(2 +),400μgL〜(-1))和镉(Cd〜(2 +),200μgL〜(-1))的混合物中长达48小时,分别在5℃和20℃的两个温度下生成敌敌畏(DDVP,100μgL〜(-1))和西维因(100μgL〜(-1))。在暴露0、6、12、24和48小时后收集样品,并在g,消化腺,地幔+肌肉和整个软组织中分析AChE活性和金属浓度(如果适用)。在敌敌畏处理中,主要在g中,观察到非常强的AChE抑制作用。西维因和金属引起短期抑制作用。注意到两个温度之间的AChE活性差异很大。特别是,金属在20℃时的累积速度比在5℃时要快得多,尤其是在g中。在AChE活性和金属浓度之间未发现相关性。 short是短期研究中AChE活性分析的最佳组织。

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