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首页> 外文期刊>Journal of environmental science and health >Mercaptobenzothiazole-on-gold Organic Phase Biosensor Systems: 1. Enhanced Organosphosphate Pesticide Determination
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Mercaptobenzothiazole-on-gold Organic Phase Biosensor Systems: 1. Enhanced Organosphosphate Pesticide Determination

机译：巯基苯并噻唑-金有机相生物传感器系统：1.增强的有机磷酸酯农药测定

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This paper reports the construction of the gold/mercaptobenzothiazole/polyaniline/acetylcholinesterase/polyvinylacetate (Au/ MBT/PANI/AChE/PVAc) thick-film biosensor for the determination of certain organophosphate pesticide solutions in selected aqueous organic solvent solutions. The Au/MBT/PANI/AChE/PVAc electrocatalytic biosensor device was constructed by encapsulating acetylcholinesterase (AChE) enzyme in the PANI polymer composite, followed by the coating of poly(vinyl acetate) (PVAc) on top to secure the biosensor film from disintegration in the organic solvents evaluated. The electroactive substrate called acetylth-iocholine (ATCh) was employed to provide the movement of electrons in the amperometric biosensor. The voltammetric results have shown that the current shifts more anodically as the Au/MBT/PANI/AChE/PVAc biosensor responded to successive acetylthio-choline (ATCh) substrate addition under anaerobic conditions in 0.1 M phosphate buffer, KCl (pH 7.2) solution and aqueous organic solvent solutions. For the Au/MBT/PANI/AChE/PVAc biosensor, various performance and stability parameters were evaluated. These factors include the optimal enzyme loading, effect of pH, long-term stability of the biosensor, temperature stability of the biosensor, the effect of polar organic solvents, and the effect of non-polar organic solvents on the amperometric behavior of the biosensor. The biosensor was then applied to detect a series of 5 organophosphorous pesticides in aqueous organic solvents and the pesticides studied were parathion-methyl, malathion and chlorpyrifos. The results obtained have shown that the detection limit values for the individual pesticides were 1.332 nM (parathion-methyl), 0.189 nM (malathion), 0.018 nM (chlorpyrifos).
机译：本文报道了金/巯基苯并噻唑/聚苯胺/乙酰胆碱酯酶/聚乙酸乙烯酯（Au / MBT / PANI / AChE / PVAc）厚膜生物传感器的构建，用于测定某些有机溶剂水溶液中的某些有机磷酸盐农药溶液。 Au / MBT / PANI / AChE / PVAc电催化生物传感器装置是通过将乙酰胆碱酯酶（AChE）封装在PANI聚合物复合材料中，然后在顶部涂覆聚乙酸乙烯酯（PVAc）来确保生物传感器膜免于崩解而构建的。在评估的有机溶剂中。称为乙酰碘胆碱（ATCh）的电活性底物用于在安培生物传感器中提供电子的运动。伏安法结果表明，随着Au / MBT / PANI / AChE / PVAc生物传感器在无氧条件下在0.1 M磷酸盐缓冲液，KCl（pH 7.2）溶液和厌氧条件下连续添加乙酰硫代胆碱（ATCh）底物时，电流的阳极移位更加明显。有机溶剂水溶液。对于Au / MBT / PANI / AChE / PVAc生物传感器，评估了各种性能和稳定性参数。这些因素包括最佳酶负载，pH值的影响，生物传感器的长期稳定性，生物传感器的温度稳定性，极性有机溶剂的影响以及非极性有机溶剂对生物传感器的安培行为的影响。然后将生物传感器应用于在水性有机溶剂中检测一系列5种有机磷农药，所研究的农药为甲基对硫磷，马拉硫磷和毒死rif。获得的结果表明，每种农药的检测极限值为1.332 nM（甲基对硫磷），0.189 nM（马拉硫磷），0.018 nM（毒死rif）。

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