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首页> 外文期刊>Journal of environmental science and health >Festuca arundinacea, glutathione S-transferase and herbicide safeners: A preliminary case study to reduce herbicidal pollution
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Festuca arundinacea, glutathione S-transferase and herbicide safeners: A preliminary case study to reduce herbicidal pollution

机译:Festuca arundinacea,谷胱甘肽S-转移酶和除草剂安全剂:减少除草剂污染的初步案例研究

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摘要

The expression of glutathione S-transferase (GST) activity in Festuca arundinacea was investigated in response to the following herbicide safeners: benoxacor, cloquintocet-mexyl, fenchlorazol-ethyl, fenclorim, fluxofenim and oxabetrinil. All the above compounds enhanced the GST activity tested towards the "model" substrate 1-chloro-2,4-dinitrobenzene (CDNB). Assays of GST activity towards the herbicides terbuthylazine (N~2-tert-butyl-6-chloro-N~4-ethyl-1,3,5-triazine-2,4-diamine) and butachlor (N-butoxymethyl-2-chloro-2',6'-diethylacetanilide) as substrates also showed the ability of the safeners to enhance the enzyme activity towards both these herbicides, with the exception of cloquintocet-mexyl for the enzyme activity towards butachlor. As a consequence of the above effects at a macro-scale level, decreased herbicide accumulation and persistence were ascertained in response to the addition of the safener benoxacor to both terbuthylazine and butachlor treatments. These results are discussed in terms of capacity of benoxacor to induce herbicide detoxification in Festuca arundinacea with a view to utilizing them in reducing herbicide pollution.
机译:响应以下除草剂安全剂,研究了五味子中谷胱甘肽S-转移酶(GST)活性的表达:苯甲沙星,克霉菌素-甲苯基,苯甲唑唑-乙基,苯氯林定,fluxofenim和oxabetrinil。所有上述化合物均增强了对“模型”底物1-氯-2,4-二硝基苯(CDNB)的GST活性。 GST对除草剂叔丁嗪(N〜2-叔丁基-6-氯代-N〜4-乙基-1,3,5-三嗪-2,4-二胺)和丁草胺(N-丁氧基甲基-2-作为底物的氯(2',6'-二乙基乙酰苯胺)也显示出安全剂增强对这两种除草剂的酶活性的能力,但对喹a啶酮的酶活性除外。由于上述作用在宏观水平上的结果,确定在对叔丁嗪和丁草胺处理中加入更安全的苯氧沙星后,除草剂的积累和持久性降低。讨论了这些结果,以贝诺沙克在Festuca arundinacea中诱导除草剂解毒的能力,以期将其用于减少除草剂污染。

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