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首页> 外文期刊>Journal of environmental science and health >In Vitro Approaches To Assess Bioavailability And Human Gastrointestinal Mobilization Of Food-borne Polychlorinated Biphenyls (pcbs)
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In Vitro Approaches To Assess Bioavailability And Human Gastrointestinal Mobilization Of Food-borne Polychlorinated Biphenyls (pcbs)

机译：评估食源性多氯联苯（pcbs）的生物利用度和人体胃肠道动员的体外方法

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摘要

This study reports on the potential for gastrointestinal (GI) mobilization and bioavailability of food-borne PCBs in humans. The development and validation of a GI simulator and operational protocols, developed in compliance with the requirements of German DIN 19738 risk assessment test procedure, are presented. Food, naturally contaminated with PCBs, was homogenized with simulated saliva fluid and shaken in the GI simulator with simulated gastric fluids (containing pepsin, mucine) for 2 h at 37℃. Afterwards, the simulated intestinal fluids (containing pepsin, mucine, trypsin, pancreatin, bile) were added and the mixture shaken for a further 6 h prior to centrifugation and filtration using Buchner funnels to separate the undigested GI residues from GI fluids. PCBs were recovered from GI residues and fluids by Soxhlet and liquid-liquid extraction respectively, cleaned up using silica-SFE, and analyzed by gas chromatography mass spectrometry detection (GC-MSD). Detailed studies with fish indicate variations in mobilization and bioavailability of ∑PCBs (28, 52, 101, 118, 153, 138 and 180). For example, the bioavailable fractions (fractions mobilized) in mackerel, salmon, crab and prawn were 0.77, 0.60, 0.54, and 0.72 respectively of the ∑PCBs initially present in these food samples. The bioavailable fraction was dependent on the physicochemical characteristics of the PCBs. In mackerel bioavailable fractions for individual PCB congeners ranged from 0.47-0.82, from 0.30-0.70 in salmon, 0.44-0.64 in crab and in prawn from 0.47-0.77. Future studies will focus on understanding better, the variability in bioavailable fractions to be expected for different foodstuffs, in addition to tissue culture techniques using human gut cell lines to investigate a simultaneous mobilization and absorption of food-borne PCBs.
机译：这项研究报告了食源性多氯联苯在人体中可能会引起胃肠道（GI）动员和生物利用度。介绍了符合德国DIN 19738风险评估测试程序要求的GI模拟器和操作规程的开发和验证。将自然污染了多氯联苯的食物与模拟唾液一起匀浆，并在GI模拟机中与模拟胃液（含胃蛋白酶，鼠类胃液）一起在37℃摇动2小时。之后，加入模拟的肠液（包含胃蛋白酶，粘蛋白，胰蛋白酶，胰酶，胆汁），并将混合物再振摇6小时，然后使用Buchner漏斗进行离心和过滤，以将未消化的GI残留物从GI液中分离出来。通过索氏提取法和液-液提取法分别从胃肠道残留物和液体中回收多氯联苯，使用硅胶-SFE进行净化，然后通过气相色谱质谱检测（GC-MSD）进行分析。对鱼类的详细研究表明，∑PCBs的动员和生物利用度存在差异（28、52、101、118、153、138和180）。例如，鲭鱼，鲑鱼，螃蟹和虾中的生物利用度分数（移动的分数）分别是这些食物样品中最初存在的∑PCB的0.77、0.60、0.54和0.72。生物利用度取决于PCB的理化特性。在单个多氯联苯同类物中鲭鱼的生物可利用分数范围为0.47-0.82，鲑鱼为0.30-0.70，螃蟹为0.44-0.64，虾为0.47-0.77。未来的研究将侧重于更好地理解不同食品的生物可利用成分的可变性，以及使用人类肠道细胞系研究同时动员和吸收食源性PCB的组织培养技术。

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