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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >The cytotoxicity of organophosphate flame retardants on HepG2, A549 and Caco-2 cells
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The cytotoxicity of organophosphate flame retardants on HepG2, A549 and Caco-2 cells

机译：有机磷酸酯阻燃剂对HepG2，A549和Caco-2细胞的细胞毒性

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摘要

In order to elucidate the cytotoxicity of organophosphate flame retardants (OPFRs), three human in vitro models, namely the HepG2 hepatoma cells, the A549 lung cancer cells and the Caco-2 colon cancer cells, were chosen to investigate the toxicity of triphenyl phosphate (TPP), tributylphosphate (TBP), tris(2-butoxyexthyl) phosphate (TBEP) and tris (2-chloroisopropyl) phosphate (TCPP). Cytotoxicity was assayed in terms of cell viability, DNA damage status, reactive oxygen species (ROS) level and lactate dehydrogenase (LDH) leakage. The results showed that all these four OPFRs could inhibit cell viability, overproduce ROS level, induce DNA lesions and increase the LDH leakage. In addition, the toxic effects of OPFRs in Caco-2 cells were relatively severer than those in HepG2 and A549 cells, which might result from some possible mechanisms apart from oxidative stress pathway. In conclusion, TBP, TPP, TBEP and TCPP could induce cell toxicity in various cell lines at relatively high concentrations as evidenced by suppression of cell viability, overproduction of ROS, induction of DNA lesions and increase of LDH leakage. Different cell types seemed to have different sensitivities and responses to OPFRs exposure, as well as the underlying potential molecular mechanisms.
机译：为了阐明有机磷酸酯阻燃剂（OPFRs）的细胞毒性，选择了三种人类体外模型，即HepG2肝癌细胞，A549肺癌细胞和Caco-2结肠癌细胞，以研究磷酸三苯酯的毒性（ TPP），磷酸三丁酯（TBP），磷酸三（2-丁氧基乙基）酯（TBEP）和磷酸三（2-氯异丙基）酯（TCPP）。根据细胞活力，DNA损伤状态，活性氧（ROS）水平和乳酸脱氢酶（LDH）泄漏测定细胞毒性。结果表明，所有这四种OPFRs都可以抑制细胞活力，过量产生ROS水平，诱导DNA损伤并增加LDH的泄漏。此外，OPFRs在Caco-2细胞中的毒性作用比在HepG2和A549细胞中更为严重，这可能是由于氧化应激途径以外的某些可能机制引起的。总之，TBP，TPP，TBEP和TCPP可以在较高浓度下诱导各种细胞系的细胞毒性，如细胞活力的抑制，ROS的过量产生，DNA损伤的诱导和LDH泄漏的增加所证明。不同的细胞类型似乎对OPFRs暴露具有不同的敏感性和反应，以及潜在的潜在分子机制。

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  • 作者

    An Jing; Hu Jingwen; Shang Yu; Zhong Yufang; Zhang Xinyu; Yu Zhiqiang;

  • 作者单位

    Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China;

    Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China;

    Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China;

    Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China;

    Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China;

    Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou, Guangdong, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    A549; Caco-2; cytotoxicity; HepG2; organophosphate flame retardants;

    机译：A549;Caco-2;细胞毒性;HepG2;有机磷酸酯阻燃剂;

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