...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Environmental Science and Health >Molecular and eco-physiological characterization of arsenic (As)-transforming Achromobacter sp. KAs 3-5~T from As-contaminated groundwater of West Bengal, India
【24h】

Molecular and eco-physiological characterization of arsenic (As)-transforming Achromobacter sp. KAs 3-5~T from As-contaminated groundwater of West Bengal, India

机译：砷（As）转化无色杆菌sp。的分子和生态生理学表征。来自印度西孟加拉邦受砷污染的地下水中的KAs 3-5〜T

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular and eco-physiological characterization of arsenic (As)-transforming and hydrocarbon-utilizing Achromobacter type strain KAs 3-5(T) has been investigated in order to gain an insight into As-geomicrobiology in the contaminated groundwater. The bacterium is isolated from As-rich groundwater of West Bengal, India. Comparative 16S rRNA gene sequence phylogenetic analysis confirmed that the strain KAs 3-5(T) is closely related to Achromobacter mucicolens LMG 26685(T) (99.17%) and Achromobacter animicus LMG 26690(T) (99.17%), thus affiliated to the genus Achromobacter. Strain KAs 3-5(T) is nonflagellated, mesophilic, facultative anaerobe, having a broad metabolic repertoire of using various sugars, sugar-/fatty acids, hydrocarbons as principal carbon substrates, and O-2, NO3-, NO2-, and Fe3+ as terminal electron acceptors. Growth with hydrocarbons led to cellular aggregation and adherence of the cells to the hydrocarbon particles confirmed through electron microscopic observations. The strain KAs 3-5(T) showed high As resistance (MIC of 5mM for As3+, 25mM for As5+) and reductive transformation of As5+ under aerobic conditions while utilizing both sugars and hydrocarbons. Molecular taxonomy specified a high genomic GC content (65.5mol %), ubiquinone 8 (UQ-8) as respiratory quinone, spermidine as predominant polyamine in the bacterium. The differential presence of C-12:0, C-14:0 2-OH, C-18:1 7c, and C (14:0) iso 3-OH/ C-16:1 iso fatty acids, phosphatidylglycerol (PG), phosphatidylcholine (PC), two unknown phospholipid (PL1, PL2) as polar lipids, low DNA-DNA relatedness (33.0-41.0%) with the Achromobacter members, and unique metabolic capacities clearly indicated the distinct genomic and physiological properties of strain KAs 3-5(T) among known species of the genus Achromobacter. These findings lead to improve our understanding on metabolic flexibility of bacteria residing in As-contaminated groundwater and As-bacteria interactions within oligotrophic aquifer system.
机译：为了对被污染的地下水中的砷地质微生物学有深入的了解，已对砷（As）转化和利用烃的无色杆菌型菌株KAs 3-5（T）的分子和生态生理特性进行了研究。该细菌是从印度西孟加拉邦富含As的地下水中分离出来的。比较16S rRNA基因序列的系统发育分析证实，KAs 3-5（T）菌株与粘液无色杆菌LMG 26685（T）（99.17％）和无色无色杆菌LMG 26690（T）（99.17％）密切相关。无色杆菌属。 KAs 3-5（T）菌株是无鞭毛的，嗜温的兼性厌氧菌，具有广泛的代谢谱，使用各种糖，糖/脂肪酸，碳氢化合物作为主要碳底物，以及O-2，NO3-，NO2-和Fe3 +作为末端电子受体。碳氢化合物的生长导致细胞聚集并通过电子显微镜观察证实细胞粘附于碳氢化合物颗粒。 KAs 3-5（T）菌株显示出高抗砷性（As3 +的MIC为5mM，As5 +的MIC为25mM），并且在好氧条件下同时利用糖和碳氢化合物对As5 +进行还原转化。分子分类学确定了高基因组GC含量（65.5mol％），泛醌8（UQ-8）是呼吸醌，亚精胺是细菌中的主要多胺。 C-12：0，C-14：0 2-OH，C-18：1 7c和C（14：0）异3-OH / C-16：1异脂肪酸，磷脂酰甘油（PG）的差异存在），磷脂酰胆碱（PC），两种未知的磷脂（PL1，PL2）作为极性脂质，与无色杆菌成员的低DNA-DNA相关性（33.0-41.0％）和独特的代谢能力清楚地表明了KA菌株的独特基因组和生理特性在无色杆菌属的已知物种中为3-5（T）。这些发现使我们对生活在砷污染地下水中的细菌的代谢灵活性以及贫营养含水层系统中的细菌相互作用产生了更好的理解。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号