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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Modelling of growth kinetics of isolated Pseudomonas sp. and optimisation of parameters for enhancement of xanthine oxidoreductase production by statistical design of experiments
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Modelling of growth kinetics of isolated Pseudomonas sp. and optimisation of parameters for enhancement of xanthine oxidoreductase production by statistical design of experiments

机译：分离的假单胞菌的生长动力学建模。统计设计的实验方法和参数的优化，以提高黄嘌呤氧化还原酶的生产

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摘要

This report presents the substrate inhibitory effect of xanthine (XN) on microbial growth and optimisation of effective parameters to achieve high enzyme activity of xanthine oxidoreductase (XOR) through statistical design. Three efficient isolated strains (Pseudomonas aeruginosa CEBP1 and CEBP2, Pseudomonas sp. CEB1G) were screened for growth kinetic studies. Substrate inhibitory models (eg. Aiba, Edward) could explain the growth kinetics of CEBP1, CEBP2 and CEB1G very well with various initial [XN] (S-0), e.g., 0.1-35 g L-1. Highest XOR activity was obtained at stationary phase when biomass yield was high. Highest catalytic efficiency (k(cat)/K-M) of XOR was obtained by CEBP1 at optimum specific growth rate of 0.082 h(-1) and biomass yield of 0.196 g g(-1) at S-0 = 5 g L-1. The effects of S-0, pH and temperature were studied by Box-Behnken experimental design to evaluate the interactive effects of the significant variables influencing XOR production by CEBP1. ANOVA with high correlation coefficient (R-2 0.99) and lower 'Prob F'value ( 0.05) validated the second order polynomial model for the enzyme production. The highest XOR activity of 31.2 KU min(-1) mg(-1) was achieved by CEBP1 under optimised conditions (35 degrees C; S-0=5 g L-1; pH = 7.0) as compared to any report in literature. A sevenfold substrate affinity of the enzyme was observed after purification.
机译：本报告介绍了黄嘌呤（XN）对微生物生长的底物抑制作用，并通过统计设计优化了实现黄嘌呤氧化还原酶（XOR）高酶活性的有效参数。筛选了三种有效的分离菌株（铜绿假单胞菌CEBP1和CEBP2，假单胞菌sp。CEB1G）进行生长动力学研究。底物抑制模型（例如Aiba，Edward）可以很好地解释CEBP1，CEBP2和CEB1G在各种初始[XN]（S-0）（例如0.1-35 g L-1）下的生长动力学。当生物质产量高时，在固定相获得最高的XOR活性。通过CEBP1在S-0 = 5 g L-1时的最佳比生长速率为0.082 h（-1）和生物量产量为0.196 g g（-1）时，CEBP1获得了最高的XOR催化效率（k（cat）/ K-M）。通过Box-Behnken实验设计研究了S-0，pH和温度的影响，以评估影响CEBP1产生XOR的重要变量的相互作用。相关系数高（R-2> 0.99）和'Prob> F'值较低（<0.05）的ANOVA验证了酶生产的二阶多项式模型。与文献中的任何报告相比，CEBP1在最佳条件下（35摄氏度； S-0 = 5 g L-1； pH = 7.0）通过CEBP1实现了最高的31.2 KU min（-1）mg（-1）的XOR活性。纯化后观察到该酶的七倍底物亲和力。

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