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首页> 外文期刊>Journal of Environmental Science and Health >Effects of ultrasonic pretreatment on quantity and composition of bacterial DNA recovered from granular activated carbon used for drinking water treatment
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Effects of ultrasonic pretreatment on quantity and composition of bacterial DNA recovered from granular activated carbon used for drinking water treatment

机译：超声波预处理对饮用水处理用颗粒活性炭回收细菌DNA数量和组成的影响

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摘要

Effects of ultrasonic pretreatment on bacterial DNA recovery from granular activated carbon (GAC) were investigated. GAC (Calgon F400), biologically activated, was sampled from an actual drinking water plant. Different ultrasonic energy densities (0-400 J·cm~(-3)) were applied with agitation (250 rpm for 30 min), and recovered bacterial DNA was quantified using quantitative PCR. Energy density was linearly correlated with the concentration of carbon fines produced from GAC during ultrasonication. Ultrasonication alone had no effect on DNA recovery at ≤60 J·cm~(-3), but a strongly adverse effect at ＞67 J·cm~(-3) due to the produced carbon fines. Agitation along with ultrasonication strongly enhanced the bacterial DNA recovery when ≤40 J·cm~(-3) was applied, although it did not affect the production of carbon fines. Ribosomal tag pyrosequencing was used to compare recovered bacterial communities (0, 20 and 30 J·cm~(-3) with or without agitation). Ultrasonication allowed for obtaining a more diverse and richer bacterial community from GAC, compared with the control. Agitation did not show a positive effect on community organization (richness and diversity). Consistently, canonical correspondence analysis indicated that the energy density was associated with the relative abundances of particular bacterial members (P ＜ 0.05), while agitation did not. Correspondence analysis revealed that the recovered bacterial communities were grouped according to the applied energy densities. In conclusion, ultrasonication and agitation influence the recovered DNA in quality and quantity, respectively, and carbon fines as a by-product by ultrasonication interfere with the DNA recovery.
机译：研究了超声波预处理对从颗粒状活性炭（GAC）回收细菌DNA的影响。 GAC（Calgon F400）具有生物活性，是从一家实际的饮用水厂中取样的。搅拌（250 rpm，30分钟）施加不同的超声能量密度（0-400 J·cm〜（-3）），并使用定量PCR定量回收的细菌DNA。能量密度与超声过程中从GAC产生的碳粉的浓度线性相关。单独的超声处理对≤60J·cm〜（-3）的DNA回收率没有影响，但由于产生的碳粉，在大于67 J·cm〜（-3）的情况下有强烈的不利影响。当应用≤40J·cm〜（-3）时，搅拌和超声处理可大大提高细菌DNA的回收率，尽管它不会影响碳粉的产生。核糖体标签焦磷酸测序用于比较回收的细菌群落（0，20和30 J·cm〜（-3）搅拌或不搅拌。与对照相比，超声处理可以从GAC获得更多样化和更丰富的细菌群落。躁动并没有对社区组织（丰富性和多样性）产生积极影响。一致地，规范对应分析表明，能量密度与特定细菌成员的相对丰度相关（P ＜0.05），而搅动则不相关。对应分析表明，回收的细菌群落根据所施加的能量密度进行分组。总之，超声处理和搅拌会分别影响回收的DNA的质量和数量，而作为超声副产物的碳粉会干扰DNA的回收。

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  • 来源
    《Journal of Environmental Science and Health》 |2014年第6期|609-616|共8页
  • 作者

    TAE GWAN KIM; SUN-HYE KIM; KYUNG-SUK CHO;

  • 作者单位

    Global Top5 Research Program, Department of Environmental Science and Engineering, Ewha Womans University, Daehyun-dong,Seodaemun-gu, Seoul, Republic of Korea;

    Global Top5 Research Program, Department of Environmental Science and Engineering, Ewha Womans University, Daehyun-dong,Seodaemun-gu, Seoul, Republic of Korea;

    Global Top5 Research Program, Department of Environmental Science and Engineering, Ewha Womans University, Daehyun-dong,Seodaemun-gu, Seoul, Republic of Korea,Department of En-vironmental Science and Engineering, Ewha Womans Univer-sity, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Drinking water treatment; ultrasonication; granular activated carbon; bacterial community; DNA recovery;

    机译：饮用水处理;超声处理颗粒状活性炭细菌群落;DNA回收;

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