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首页> 外文期刊>Journal of Environmental Science and Health >Application of radio-immunoassays to assess the fate of estrogen EDCs in full scale wastewater treatment plants
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Application of radio-immunoassays to assess the fate of estrogen EDCs in full scale wastewater treatment plants

机译:放射免疫法在评估大型污水处理厂中雌激素EDC命运方面的应用

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Wastewater effluents have been documented as major contributors of hormone endocrine disrupting compounds (EDCs) in to the aquatic ecosystem. The need for rapid, simple and cost effective methods to detect these EDCs has increased. The use of Radio-immunoassays (RIA) were assessed to determine the fate of estradiol in a laboratory batch test and the three natural estrogens (estrone (El), estradiol (E2) and estriol (E3)) in wastewater treatment plants (WWTPs) with different types of configurations. Precision of the RIAs were done using intra-assay and inter-assay validations. The E2 intra-assay variation was <8% and inter-assay variation was <11% for standards 1 to 6. El RIA showed less than 8% for both the intra-assay and inter-assay variations. E3 RIA showed extremely good variations with both the intra and inter-assay variations being below <8% for all standards. The lab scale investigation showed a 94% reduction in E2 after 5 h and after 10 h both E2 and El were no longer detected. The simple activated sludge process, the biological nutrient removal (BNR) activated sludge process and the oxidation pond had final effluent concentrations of 10.75, 5.96 and 25.48 pg E2/mL respectively; 20.80, 9.30 and 46.55 pg E1/mL, respectively, and 0.12, 0.07 and 0.17 ng E3/mL, respectively. Thus far findings indicated that the RIA can be employed as a rapid technique for detection of natural estrogens in water. Results indicate that these potential problematic hormone EDCs are still present in final wastewater effluents that are discharged in to South African aquatic sources.
机译:废水已被记录为水生生态系统中激素内分泌干扰化合物(EDC)的主要贡献者。快速,简单和经济有效的方法来检测这些EDC的需求在增加。在实验室分批测试中评估了放射免疫测定(RIA)的使用以确定雌二醇的命运以及废水处理厂(WWTP)中的三种天然雌激素(雌酮(El),雌二醇(E2)和雌三醇(E3))的命运。具有不同类型的配置。使用批内和批间验证来完成RIA的精确度。对于标准品1至6,E2批内差异小于8%,批间差异小于11%。El RIA批内和批间差异均小于8%。 E3 RIA表现出非常好的变化,所有标准品的批内和批间差异均小于<8%。实验室规模调查显示,5小时后和10小时后,E2降低了94%,E2和E1均不再被检测到。简单的活性污泥法,生物营养物去除(BNR)活性污泥法和氧化池的最终废水浓度分别为10.75、5.96和25.48 pg E2 / mL。分别为20.80、9.30和46.55 pg E1 / mL,分别为0.12、0.07和0.17 ng E3 / mL。迄今为止的发现表明,RIA可以用作检测水中天然雌激素的快速技术。结果表明,这些潜在的有问题的激素EDC仍然存在于最终排放到南非水生源的废水中。

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