Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities

TAE GWAN KIM; TAEWOO YI; KYUNG-SUK CHO

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Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities

机译：使用具有多个探针位点的人工DNA作为参考DNA模板进行实时定量PCR检测产甲烷菌群落

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摘要

Isolation of reference DNA templates for quantitative real-time PCR assays is an expensive, labor-intensive and time-consuming process if they are not readily available. Two artificial DNA templates with multiple probe sites were designed for quantifying methanogens and their 10 subgroups, based on the methyl coenzyme M reductase gene (mcrA). Their standards were comparable to each other. PCR amplification efficiencies (cycle vs. cumulative fluorescence) of the artificial DN As were also comparable to those of the observed methanogen groups from anaerobic digesters. The artificial templates can be alternatives to the actual references.

机译：如果不容易获得用于实时荧光定量PCR检测的参考DNA模板，则该过程昂贵，费力且耗时。基于甲基辅酶M还原酶基因（mcrA），设计了两个带有多个探针位点的人工DNA模板，用于量化产甲烷菌及其10个亚组。他们的标准可以相互比较。人工DNA的PCR扩增效率（周期与累积荧光）也与厌氧消化池中观察到的产甲烷菌组相当。人工模板可以替代实际参考。

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来源
《Journal of Environmental Science and Health》 |2013年第4期|417-421|共5页
作者
TAE GWAN KIM; TAEWOO YI; KYUNG-SUK CHO;
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作者单位

Department of Environmental Science and Engineering, Ewha Womans University, Seodaemun-gu, Seoul, Korea;

Department of Environmental Science and Engineering, Ewha Womans University, Seodaemun-gu, Seoul, Korea;

Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Quantitative PCR; artificial DNA; methanogens; mcrA;

机译：定量PCR;人工DNA产甲烷菌微机;

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Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities

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