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首页> 外文期刊>Journal of Environmental Science and Health >Biochemical signature assay for use in a biosensor platform to detect bacteria in drinking water biofilms
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Biochemical signature assay for use in a biosensor platform to detect bacteria in drinking water biofilms

机译：用于生物传感器平台的生化特征分析，用于检测饮用水生物膜中的细菌

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摘要

The objective of the study was to identify the enzymatic-biochemical (enz-bio) signatures of Escherichia coli and Salmonella for rapid detection of these bacteria in drinking water biofilms. The relative potency of lipophilic, glucosidic, and proteolytic activities in biofilms containing single bacterial species and mixture of different bacterial was used to identify the enz-bio signatures of Escherichia coli and Salmonella. The enz-bio signatures identified were: Lipophilic ＜ Glucosidic ＜ Proteolytic (for Escherichia coli); and Glucosidic = Lipophilic ＜ Proteolytic (for Salmonella). The enz-bio assays were performed sequentially for detecting Escherichia coli and Salmonella in pure and mixed biofilm cultures formed on the coupons incubated in a batch reactor. The results obtained were substantiated by culture-based assays indicating comparable data. The enz-bio sensing method described here is a proof of principle and the results of this study provide a platform for the fabrication of a biosensor for bacterial detection in biofilms. The detection time required for the biosensor platform versus culture methods ranged from 10 to 120 min and 24 to 48 h, respectively.
机译：该研究的目的是鉴定大肠杆菌和沙门氏菌的酶生化特征（enz-bio），以快速检测饮用水生物膜中的这些细菌。在包含单个细菌物种和不同细菌混合物的生物膜中，亲脂性，糖苷性和蛋白水解活性的相对效力用于鉴定大肠杆菌和沙门氏菌的恩兹生物特征。鉴定的enz-bio特征为：亲脂性＜糖苷＜蛋白水解（对于大肠杆菌）；和糖苷=亲脂性＜蛋白水解（对于沙门氏菌）。依次进行enz-bio检测，以检测在分批反应器中温育的挂片上形成的纯生物膜和混合生物膜培养物中的大肠杆菌和沙门氏菌。所得结果通过基于培养物的分析证实，表明可比较的数据。这里描述的enz-bio传感方法是原理的证明，这项研究的结果为制造用于生物膜中细菌检测的生物传感器提供了平台。生物传感器平台相对于培养方法所需的检测时间分别为10至120分钟和24至48小时。

著录项

  • 来源
    《Journal of Environmental Science and Health》 |2013年第8期|925-932|共8页
  • 作者

    MOHAMAD ELZEIN; ABSAR ALUM; MORTEZA ABBASZADEGAN;

  • 作者单位

    Civil, Environmental and Sustainable Engineering, National Science Foundation Water & Environmental Technology Center, Arizona State University, Tempe, Arizona, USA;

    Civil, Environmental and Sustainable Engineering, National Science Foundation Water & Environmental Technology Center, Arizona State University, Tempe, Arizona, USA;

    Civil, Environmental and Sustainable Engineering, National Science Foundation Water & Environmental Technology Center, Arizona State University, Tempe, Arizona, USA Department of Civil, Environmental and Sustainable Engineering, Arizona State University, Tempe, AZ 85287-5306, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biofilm; Biosensor; Biochemical signature; Escherichia coli; Salmonella;

    机译：生物膜生物传感器生化签名;大肠杆菌;沙门氏菌;

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