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首页> 外文期刊>Journal of Environmental Science and Health >Antibacterial effect of chronic exposure of low concentration ZnO nanoparticles on E. coli
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Antibacterial effect of chronic exposure of low concentration ZnO nanoparticles on E. coli

机译：低浓度ZnO纳米颗粒长期暴露于大肠杆菌的抗菌作用

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摘要

The toxicity effect due to chronic exposure of ZnO nanoparticles (NPs) was systematically studied by repeatedly treating different lower concentrations of ZnO nanoparticles with culture media of E. coli strain. The chronic exposure of ZnO NPs of concentrations below minimum inhibitory concentration (MIC) exhibited higher toxicity than the single exposure of higher concentrations. Most striking result was 57% inhibition of growth corresponding to chronic exposure of 0.06 mg/mL of ZnO NPs which was two folds more than that exhibited by single exposure of 0.30 mg/mL ZnO NPs. The toxicity of ZnO NPs in E. coli was studied in the light of formation of reactive oxygen species (ROS), measured as malondialdehyde (MDA) equivalent by thiobarbituric acid-ROS (TBARS) assay, and effect of Zn dissolution from ZnO NPs. Higher inhibition of growth for the chronic exposure batches were correlated with higher ROS generation, which subsequently contributed to cause membrane lipid peroxidation, confirmed from observation of cell wall deformation by scanning electron microscopy study and energy dispersive X-ray analysis showed adherence of ZnO NPs on cell wall. The possibility of membrane lipid peroxidation was addressed by revealing in vitro oxidation of oleic acid, which is a monounsaturated fatty acid. Further in this study we have shown that the dissolution of ZnO NPs at pH 7.4 was not significant to cause Zn-induced toxicity.
机译：通过用大肠杆菌菌株的培养基反复处理不同浓度较低的ZnO纳米颗粒，系统地研究了由于ZnO纳米颗粒（NPs）的长期暴露而引起的毒性作用。浓度低于最低抑菌浓度（MIC）的ZnO NPs的长期暴露比单独暴露较高浓度的ZnO NPs具有更高的毒性。最惊人的结果是对生长的抑制为57％，这对应于0.06 mg / mL的ZnO NP的长期暴露，是单次暴露0.30 mg / mL的ZnO NP所表现出的两倍。 ZnO NPs在大肠杆菌中的毒性是根据活性氧（ROS）的形成，通过硫代巴比妥酸-ROS（TBARS）测定的丙二醛（MDA）当量以及锌从ZnO NPs溶出的影响来研究的。慢性暴露批次对生长的更高抑制作用与更高的活性氧生成有关，活性氧随后导致膜脂质过氧化，通过扫描电子显微镜研究观察到的细胞壁变形和能量分散X射线分析证实了ZnO NPs的粘附性证实了这一点。细胞壁。膜脂质过氧化的可能性通过揭示油酸的体外氧化得到解决，油酸是一种单不饱和脂肪酸。此外，在这项研究中，我们还表明，在pH 7.4下ZnO NPs的溶出并不显着引起Zn诱导的毒性。

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