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首页> 外文期刊>Journal of Environmental Science and Health >Isolation and identification of chromium-resistant bacteria: Test application for prevention of chromium toxicity in plant
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Isolation and identification of chromium-resistant bacteria: Test application for prevention of chromium toxicity in plant

机译：耐铬细菌的分离与鉴定：防止植物中铬毒性的试验应用

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摘要

The purpose of this study was to isolate chromium resistant bacteria and characterize in depth before being applied for bioremediation or construction of chromium biosesnors. One of the major objectives was to identify the most useful strains with respect to chromium removal efficiency. Chromium resistant bacteria were isolated from tannery waste samples. These were characterized through biochemical tests and antibiotic assays. Cross metal reactivity, plasmid curing and cysteine quantifications were also performed. Gene sequencing was done for all the isolates and accession numbers received after submission to National Center for Biotechnology Information (NCBI). The removal capacity was also studied in broth cultures. The results were very encouraging since six new bacteria were recognised to withstand high concentrations of chromium. Out of these six bacteria, three i.e. Enterobacter aerogenes, Aeromonas sp., Acinetobacter sp. PD 12 performed very well by removing about 99% (average) from initial 19.8 ppm of Cr(VI) from a synthetic culture media. Enterobacter aerogenes, being the most efficient strain, was used to remediate hexavalent chromium from soil of potted plants- very encouraging results were obtained. These bacteria may be exploited for bioremediation of Cr (VI) and construction of microbial sensor in future.
机译：这项研究的目的是分离出耐铬细菌，并在应用于生物修复或铬生物传感器的构造之前进行深度表征。主要目标之一是就铬的去除效率确定最有用的菌株。从制革厂废物样品中分离出耐铬细菌。这些通过生化测试和抗生素测定来表征。还进行了跨金属反应性，质粒固化和半胱氨酸定量。提交给国家生物技术信息中心（NCBI）后，对所有分离株和登录号进行基因测序。还研究了肉汤培养物中的去除能力。该结果令人鼓舞，因为公认有六种新细菌可以耐受高浓度的铬。在这六种细菌中，有三种，即产气肠杆菌，气单胞菌属，不动杆菌属。通过从合成培养基中去除最初的19.8 ppm的Cr（VI）约99％（平均值），PD 12表现非常出色。最有效的菌株是产气肠杆菌，用于从盆栽植物土壤中修复六价铬，获得了令人鼓舞的结果。这些细菌将来可能被用于Cr（VI）的生物修复和微生物传感器的构建。

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