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首页> 外文期刊>Journal of Environmental Science and Health >Assessment of the genotoxicity of heavy metals in Phaseolus vulgaris L. as a model plant system by Random Amplified Polymorphic DNA (RAPD) analysis
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Assessment of the genotoxicity of heavy metals in Phaseolus vulgaris L. as a model plant system by Random Amplified Polymorphic DNA (RAPD) analysis

机译：通过随机扩增多态性DNA（RAPD）分析评估菜豆作为模型植物系统中重金属的遗传毒性

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摘要

Impact assessments of environmental pollutants are important in eco-genotoxicology. A random amplified polymorphic DNA (RAPD) technique was used to detect genotoxicity-induced DNA damage in Phaseolus vulgaris L. from heavy metals at two different concentrations. The results from six 10-base pair (bp) random RAPD primers with 60-70% GC content used, showed a total of 295 RAPD fragments of 700-4000 bp in molecular size in the seedlings of untreated and treated samples, of which only 163 fragments were polymorphic. Polymorphisms became evident as the disappearance and/or appearance of DNA fragments in treated samples compared to the control. A dendrogram constructed using the Numerical Taxonomy and Multivariate Analysis System (NTSYSps), showed that the control group merged with groups treated with CuSO_4·5H_2O (150 mg L~(-1)) and MnS_4·H_2O (150 mg L~(-1)) in a separate cluster. These groups were linked with all of the other samples treated with metals at concentrations of 150 mg L~(-1) and CuSO_4·5H_2O and Cd(NO_3)_2 at concentrations of 350 mg L~(-1). Finally, the samples treated with metals at concentrations of 350 mg L~' together with NiSO_4 at the concentration of 150 mg L~(-1), clustered separately. The DNA polymorphism detected by RAPD analysis offered a useful biomarker assay for the detection of toxic chemicals genotoxicity in plant model systems.
机译：环境污染物的影响评估在生态遗传毒理学中很重要。随机扩增多态性DNA（RAPD）技术用于检测遗传毒性诱导的菜豆中DNA的两种不同浓度的DNA损伤。使用六种10碱基对（bp）随机RAPD引物的结果，这些引物的GC含量为60-70％，显示未经处理和处理的样品的幼苗中共有295个分子大小为700-4000 bp的RAPD片段163个片段是多态的。与对照相比，随着处理样品中DNA片段的消失和/或出现，多态性变得明显。使用数值分类和多元分析系统（NTSYSps）构建的树状图显示，对照组与用CuSO_4·5H_2O（150 mg L〜（-1））和MnS_4·H_2O（150 mg L〜（-1）处理的组合并））在一个单独的群集中。这些组与其他所有使用浓度为150 mg L〜（-1）的金属处理过的样品以及浓度为350 mg L〜（-1）的CuSO_4·5H_2O和Cd（NO_3）_2相连。最后，分别用浓度为350 mg L''的金属和浓度为150 mg L'（-1）的NiSO_4处理的样品聚类。通过RAPD分析检测到的DNA多态性为在植物模型系统中检测有毒化学物质的遗传毒性提供了有用的生物标记测定。

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  • 来源
    《Journal of Environmental Science and Health》 |2012年第3期|p.366-373|共8页
  • 作者

    DARINKA GJORGIEVA; TATJANA KADIFKOVA-PANOVSKA; SASA MITREV; BILJANA KOVACEVIK; EMILIJA KOSTADINOVSKA; KATERINA BACEVA; TRAJCE STAFILOV;

  • 作者单位

    Faculty of Medical Sciences, Goce Delcev University, Stip, R. Macedonia;

    Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, R. Macedonia;

    Faculty of Agriculture, Goce Delcev University, Stip, R. Macedonia;

    Faculty of Agriculture, Goce Delcev University, Stip, R. Macedonia;

    Faculty of Agriculture, Goce Delcev University, Stip, R. Macedonia;

    Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, R. Macedonia;

    Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, R. Macedonia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    RAPD; genotoxic effects; phaseolus vulgaris; heavy metals;

    机译：RAPD;遗传毒性作用;菜豆重金属;

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