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首页> 外文期刊>Journal of Environmental Science and Health >Survival of infectious prions in Class B biosolids
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Survival of infectious prions in Class B biosolids

机译：B类生物固体中感染性病毒的存活

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摘要

This study developed a method for extracting infectious prions from Class B biosolids and subsequently evaluated the survival of infectious prions under the influence of mesophilic (37℃) and thermophilic (60℃) temperatures in Class B biosolids. Unlike other studies, this study utilized a scrapie cell assay to determine infectivity and quantity of infectious prions. The best method for extraction was exposing the biosolids to 4 M urea at 80℃ for 10 minutes followed by a membrane centrifugation to reduce the concentration of urea. The recovery efficiency of the infectious prions from the biosolids for this method was 17.2%. In the survival study, a 2.43-log_(10) reduction in prion infectivity was observed under mesophilic temperatures after 15 days and a 3.41-log_(10) reduction after 10 days under thermophilic conditions. The reduction of infectious prions was greater in the biosolids than the control in phosphate buffered saline, suggesting factors other than temperature were also playing a role in the loss of infectivity of the prions in the biosolids.
机译：本研究开发了一种从B类生物固体中提取感染性ions病毒的方法，然后评估了B类生物固体中嗜温（37℃）和嗜热性（60℃）温度影响下感染性病毒的存活率。与其他研究不同，该研究利用瘙痒病细胞测定法来确定感染性和感染性病毒的数量。提取的最佳方法是将生物固体在80℃下暴露于4 M尿素中10分钟，然后进行膜离心以降低尿素的浓度。该方法从生物固体中回收感染性ions病毒的效率为17.2％。在存活研究中，在嗜温条件下在中温温度下15天后观察到病毒感染性降低了2.43 log_（10），在嗜热条件下10天后观察到a病毒感染率降低了3.41-log_（10）。生物固体中感染性pr病毒的减少量大于磷酸盐缓冲盐水中的对照，表明温度以外的因素也在生物固体中the病毒的感染性丧失中起作用。

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  • 来源
    《Journal of Environmental Science and Health》 |2011年第4期|p.364-370|共7页
  • 作者

    SYREETA L. MILES; KAZUE TAKIZAWA; CHARLES P. GERBA; IAN L. PEPPER;

  • 作者单位

    Soil, Water, and Environmental Science Department, University of Arizona, Tucson, Arizona,University of Arizona, Environmental Research Laboratory, 2601 E. Airport Dr., Tucson, AZ 85756, USA;

    Soil, Water, and Environmental Science Department, University of Arizona, Tucson, Arizona;

    Soil, Water, and Environmental Science Department, University of Arizona, Tucson, Arizona;

    Soil, Water, and Environmental Science Department, University of Arizona, Tucson, Arizona;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    prions; infectivity; biosolids; sludge; survival; temperature;

    机译：ions病毒;传染性生物固体污泥;生存温度;

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