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Comparisons of Vibrio fischeri, Photobacterium phosphoreum, and recombinant luminescent using Escherichia coli as BOD measurement

机译:用大肠杆菌作为BOD测定的费氏弧菌,磷光细菌和重组发光的比较

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摘要

To shorten the time needed to measure biochemical oxygen demand (BOD) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria Vibrio fischeri, Photobacterium phosphoreum, and recombinant Escherichia coli as potential indicators of BOD in the domestic wastewaters. The results indicate that the luminescence intensities of these strains are dependent on temperature, pH, and BOD concentration. In comparison to the standard BOD_5 method, the time needed for BOD measurement can be shortened by 90, 120, and 150 min when V. fischeri, P. phosphoreum, and recombinant E. coli, respectively, are used. Recombinant E. coli can be adapted to measure BOD in domestic wastewater containing a wide range of BOD concentrations, V. fischeri is not suitable for measuring diluted wastewater, and P. phosphoreum has only a limited application in measuring concentrated wastewater. To the best of our knowledge, this is the first report in which V. fischeri, P. phosphoreum, and recombinant luminescent E. coli are compared in terms of their potential in BOD measurement systems.
机译:为了缩短测量水样中生化需氧量(BOD)所需的时间并提供水质真实状况的快速反馈,我们测试并评估了发光细菌费氏弧菌,磷光细菌和重组大肠杆菌的有效性和可靠性。大肠杆菌作为生活污水中BOD的潜在指标。结果表明,这些菌株的发光强度取决于温度,pH和BOD浓度。与标准的BOD_5方法相比,当分别使用费氏弧菌,磷青霉和重组大肠杆菌时,BOD测量所需的时间可以缩短90、120和150分钟。重组大肠杆菌可适用于测量各种BOD浓度范围的生活废水中的BOD,费氏弧菌不适合测量稀释的废水,而P.磷在测量浓缩废水中的应用有限。据我们所知,这是第一份报告,其中比较了费氏弧菌,磷杆菌和重组发光大肠杆菌在BOD测量系统中的潜力。

著录项

  • 来源
    《Journal of Environmental Science and Health》 |2010年第2期|233-238|共6页
  • 作者单位

    Department of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC;

    rnDepartment of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC;

    rnDepartment of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC;

    rnDepartment of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC;

    rnDepartment of Biological Science and Technology, China University of Science and Technology, Taipei, Taiwan, ROC;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biochemical oxygen demand; biosensor; Vibrio fischeri; Photobacterium phosphoreum; Escherichia coli;

    机译:生化需氧量;生物传感器费氏弧菌;发光细菌大肠杆菌;

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