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首页> 外文期刊>Journal of Environmental Science and Health >Construction of a genetically engineered microorganism with high tolerance to arsenite and strong arsenite oxidative ability
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Construction of a genetically engineered microorganism with high tolerance to arsenite and strong arsenite oxidative ability

机译：具有亚砷酸盐高耐受性和强亚砷酸盐氧化能力的基因工程微生物的构建

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摘要

Genetically engineered microorganisms (GEMs) have shown great potential for use in environmental bioremediation. In this study, the TTHB128 and TTHB127 genes, which encode the small and large subunits of arsentie oxidase in Thermus thermophilus HB8, respectively, were cloned into the broad-host-range vector pBBR1MCS-5 to produce the recombinant plasmid, TTHB127-pBBRlMCS-5-TTHB128. This resulted in successful construction of a GEM with high tolerance to arsenite and strong arsenite oxidative ability. Culture of the GEM in media containing arsenite for 28 h resulted in 87.6% of the arsenite being oxidized. Overall, the oxidative ability of the GEM was much stronger than that of the wild type host strain. Gentamicin was necessary to maintain the stability of the recombinant plasmid, TTHB127-pBBRlMCS-5-TTHB128, in the GEM. The oxidative ability of the GEM remained unchanged when it was grown in medium containing gentamicin (60 mg/L) for 30 growth cycles, after which its activity gradually decreased.
机译：基因工程微生物（GEM）已显示出在环境生物修复中的巨大潜力。在这项研究中，将分别编码嗜热栖热菌HB8中砷酸氧化酶的小亚基和大亚基的TTHB128和TTHB127基因克隆到宽宿主范围的载体pBBR1MCS-5中，以产生重组质粒TTHB127-pBBR1MCS- 5-TTHB128。这成功地构建了具有高砷耐受性和强砷氧化能力的GEM。在含砷的培养基中培养GEM 28小时，导致87.6％的砷被氧化。总体而言，GEM的氧化能力比野生型宿主菌株强得多。庆大霉素对于维持GEM中重组质粒TTHB127-pBBR1MCS-5-TTHB128的稳定性是必需的。当GEM在含有庆大霉素（60 mg / L）的培养基中生长30个生长周期时，其氧化能力保持不变，此后其活性逐渐降低。

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  • 来源
    《Journal of Environmental Science and Health》 |2010年第6期|P.740-745|共6页
  • 作者

    CHUNYAN YANG; LIN XU; LIMIN YAN; YANHUA XU;

  • 作者单位

    College of Environment, Nanjing University of Technology, Nanjing, PR China;

    rnCollege of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, PR China;

    rnCollege of Environment, Nanjing University of Technology, Nanjing, PR China;

    rnCollege of Environment, Nanjing University of Technology, Nanjing, PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    tolerance; arsenite; bioconversion; genetic stability; DNA; enzyme;

    机译：公差;亚砷酸盐生物转化遗传稳定性脱氧核糖核酸;酵素;

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