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首页> 外文期刊>Journal of Environmental Science and Health >Characterization of microbial communities in a pilot-scale constructed wetland using PLFA and PCR-DGGE analyses
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Characterization of microbial communities in a pilot-scale constructed wetland using PLFA and PCR-DGGE analyses

机译：使用PLFA和PCR-DGGE分析表征中试规模人工湿地中的微生物群落

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摘要

Phospholipid fatty acid (PLFA) analysis and 16S ribosomal DNA polymerase chain reaction amplification-denaturing gradient gel electrophoresis (PCR-DGGE) were used to determine microbial communities and predominant microbial populations in water samples collected from a pilot-scale constructed wetland system. This pilot-scale constructed wetland system consists of three types: subsurface-flow (SSF), surface-flow (SF) and a floating aquatic plant (FAP) system. Analysis of PLFA profiles indicated primarily eukaryotic organisms, including fungi, protozoa, and diatoms were observed in all three wetland systems. Biomarkers for Gram-negative bacteria were also detected in all samples analyzed while low proportions of biomarkers for Gram-positive bacteria were observed. Biomass content (total PFLA/sample) was highest in water samples collected from both SF and FAP system while highest metabolic activity was observed in FAP system. This is consistent with the observed highest metal removal rate in FAP system. Sequence analysis of the predominant PCR-DGGE DNA fragments showed 0.92 to 0.99 similarity indices to Beta-proteobacteria, Flavobacterium sp. GOBB3-206, Flexibacter-Cytophaga-Bacteroides group, and Gram-positive bacteria. Results suggest diverse microbial communities including microorganisms that may significantly contribute to biogeochemical elemental cycles.
机译：磷脂脂肪酸（PLFA）分析和16S核糖体DNA聚合酶链反应扩增-变性梯度凝胶电泳（PCR-DGGE）用于测定从中试规模人工湿地系统收集的水样中的微生物群落和主要微生物种群。这个中试规模的人工湿地系统包括三种类型：地下流（SSF），地表流（SF）和漂浮水生植物（FAP）系统。对PLFA谱的分析表明，在所有三个湿地系统中都主要观察到真核生物，包括真菌，原生动物和硅藻。在所有分析的样品中，革兰氏阴性菌的生物标志物也都被检测到，而革兰氏阳性菌的生物标志物比例低。从SF和FAP系统收集的水样品中，生物质含量（总PFLA /样品）最高，而在FAP系统中观察到最高的代谢活性。这与在FAP系统中观察到的最高金属去除率一致。主要PCR-DGGE DNA片段的序列分析显示与β-变形杆菌Flavobacterium sp。有0.92至0.99的相似性指数。 GOBB3-206，Flexibacter-Cytophaga-Bacteroides组和革兰氏阳性细菌。结果表明，包括可能对生物地球化学元素周期有重大贡献的微生物在内的各种微生物群落。

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