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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5
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Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5

机译：苯胺降解红球菌的分离和鉴定。菌株AN5

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摘要

A pure culture using aniline as a sole source of carbon and energy was isolated by selective enrichment culturing on a minimum salt medium (MSM) from the Nanjing Chemical Plant, Nanjing, Jiangsu Province, China. An analysis of the 16S rRNA gene sequence and morphological and physiological characteristics showed that this strain was a member of the genus Rhodococcus, and it was designated as strain AN5. Its optimal conditions for aniline biodegradation were 30℃ (pH 7.0). Due to the water insolubility of aniline, growth of strain AN5 at various aniline concentrations was studied via spectrophotometry and microcalorimetry in different culture mediums. Strain AN5 not only utilized aniline as its sole carbon and energy resources, but also degraded phenol, benzoic acid and naphthalin. Glucose, peptone and ammonium sulfate, when utilized as its secondary carbon and nitrogen sources, had no active effect on the aniline biodegradation. Such findings would be valuable in the application of strain AN5 to depurate industrial waste water.
机译：通过在中国江苏省南京市的南京化工厂的最低盐培养基（MSM）上进行选择性富集培养，分离出了以苯胺为唯一碳和能源的纯培养物。对16S rRNA基因序列及形态和生理特性的分析表明，该菌株是红球菌属的一员，被命名为AN5菌株。苯胺生物降解的最佳条件是30℃（pH 7.0）。由于苯胺的水不溶性，通过分光光度法和微量量热法在不同的培养基中研究了在各种苯胺浓度下菌株AN5的生长。 AN5菌株不仅利用苯胺作为唯一的碳和能源，而且还降解了苯酚，苯甲酸和萘。葡萄糖，蛋白ept和硫酸铵用作其次要碳源和氮源时，对苯胺的生物降解没有积极作用。这些发现对于AN5菌株净化工业废水的应用将是有价值的。

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